|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||February 01, 2019|
|Effective date (End):||January 31, 2020|
|Field of knowledge:||Health Sciences - Dentistry - Endodontics|
|Principal Investigator:||Gisele Faria|
|Home Institution:||Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil|
Pulp repair involves proliferation, migration and differentiation of progenitor cells of mesenchymal origin into osteoblats and odontoblasts-like at the site of injury, which leads to the formation of mineralized tissue. Preliminary results from our research group showed that the Citrus CPI-2 cystatin, derived Citrus sinensis (sweet orange), was able to inhibit the enzymatic activity of human cathepsins, had anti-inflammatory activity and potential to induce differentiation of undifferentiated cells into osteogenic phenotype, indicating its potential use in the modulation of cellular metabolism. The aim of the present project is to study the effect of Citrus CPI-2 phytocystatin on the proliferation, migration and differentiation of cells from human dental pulp. Specifically, we will study the cell migration through the traswell assay, cell proliferation by bromo-oxyuridine incorporation enzyme-linked immunosorbent assay (BrdU) and the gene expression of markers related to osteogenic and odontogenic differentiation through qRT-PCR.