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Osteogenic and odontogenic potential of cystatin Citrus CPI-2, derived from Citrus sinensis (sweet orange), in human dental pulp cells

Grant number: 18/24150-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2019
Effective date (End): January 31, 2020
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Gisele Faria
Grantee:Bárbara Roma Mendes
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Pulp repair involves proliferation, migration and differentiation of progenitor cells of mesenchymal origin into osteoblats and odontoblasts-like at the site of injury, which leads to the formation of mineralized tissue. Preliminary results from our research group showed that the Citrus CPI-2 cystatin, derived Citrus sinensis (sweet orange), was able to inhibit the enzymatic activity of human cathepsins, had anti-inflammatory activity and potential to induce differentiation of undifferentiated cells into osteogenic phenotype, indicating its potential use in the modulation of cellular metabolism. The aim of the present project is to study the effect of Citrus CPI-2 phytocystatin on the proliferation, migration and differentiation of cells from human dental pulp. Specifically, we will study the cell migration through the traswell assay, cell proliferation by bromo-oxyuridine incorporation enzyme-linked immunosorbent assay (BrdU) and the gene expression of markers related to osteogenic and odontogenic differentiation through qRT-PCR.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA COSTA FERNANDES, JR., CELIO; RODRIGUEZ, VICTOR MANUEL OCHOA; SOARES-COSTA, ANDREA; CIRELLI, JONI AUGUSTO; JUSTINO, DANIELA MORILHA NEO; ROMA, BARBARA; ZAMBUZZI, WILLIAN FERNANDO; FARIA, GISELE. Cystatin-like protein of sweet orange (CsinCPI-2) modulates pre-osteoblast differentiation via beta-Catenin involvement. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 32, n. 4, . (14/22689-3, 18/24150-5, 12/24278-5, 17/05784-0, 19/21807-6)

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