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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cystatin-like protein of sweet orange (CsinCPI-2) modulates pre-osteoblast differentiation via beta-Catenin involvement

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Author(s):
da Costa Fernandes, Jr., Celio [1] ; Rodriguez, Victor Manuel Ochoa [2] ; Soares-Costa, Andrea [3] ; Cirelli, Joni Augusto [4] ; Justino, Daniela Morilha Neo [3] ; Roma, Barbara [2] ; Zambuzzi, Willian Fernando [1] ; Faria, Gisele [2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Chem & Biochem, Lab Bioassays & Cell Dynam, Inst Biosci, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Restorat Dent, Araraquara, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil
[4] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 4 APR 2021.
Web of Science Citations: 0
Abstract

Phytocystatins are endogenous cysteine-protease inhibitors present in plants. They are involved in initial germination rates and in plant defense mechanisms against phytopathogens. Recently, a new phytocystatin derived from sweet orange, CsinCPI-2, has been shown to inhibit the enzymatic activity of human cathepsins, presenting anti-inflammatory potential and pro-osteogenic effect in human dental pulp cells. The osteogenic potential of the CsinCPI-2 protein represents a new insight into plants cysteine proteases inhibitors and this effect needs to be better addressed. The aim of this study was to investigate the performance of pre-osteoblasts in response to CsinCPI-2, mainly focusing on cell adhesion, proliferation and differentiation mechanisms. Together our data show that in the first hours of treatment, protein in CsinCPI-2 promotes an increase in the expression of adhesion markers, which decrease after 24 h, leading to the activation of Kinase-dependent cyclines (CDKs) modulating the transition from G1 to S phases cell cycle. In addition, we saw that the increase in ERK may be associated with activation of the differentiation profile, also observed with an increase in the B-Catenin pathway and an increase in the expression of Runx2 in the group that received the treatment with CsinCPI-2. {[}GRAPHICS] . (AU)

FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/24150-5 - Osteogenic and odontogenic potential of cystatin Citrus CPI-2, derived from Citrus sinensis (sweet orange), in human dental pulp cells
Grantee:Bárbara Roma Mendes
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/24278-5 - Identification and characterization of cysteine peptidases from Diaphorina citri, Huanglongbing disease vector, and studies of interaction between cysteine peptidases and citrus cystatins
Grantee:Andrea Soares da Costa Fuentes
Support Opportunities: Regular Research Grants
FAPESP's process: 17/05784-0 - Effect of phytocystatin Citrus CPI-2 on the proliferation, migration and differentiation of cells from human apical papilla and dental pulp
Grantee:Gisele Faria
Support Opportunities: Regular Research Grants
FAPESP's process: 19/21807-6 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Célio Junior da Costa Fernandes
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)