Label-free electrochemical capacitive biosensors for disease diagnosis

• Grant number: 18/26273-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2019
• End date: May 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Paulo Roberto Bueno
• Grantee: Beatriz Lucas Garrote
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 17/24839-0 - Nanoelectronics and nanoscale electrochemistry: fundaments and applications, AP.TEM

Abstract

Biomedicine research is directing its effort to achieve fast, simple, point-of-care analytical devices for disease diagnosis in order to reduce the analysis time and cost of the current diagnostic methods. Label-free electrochemical biosensor based on redox capacitance fulfils those characteristics. It has been widely proved its capability in biosensing at low concentrations. The aim of this project is to apply this sensitive and reliable technology for the detection of Flavivirus infections that have become a health-threating in many countries, such as Brazil. Diagnosis will be performed by the detection of the biomarkers NS1 and EDIII. Also, it will be analysed whether it is a recurrent infection by the quantification of IgG and IgM; and Alzheimer's Disease, the most common type of dementia. In the latter case, diagnosis will be performed by biomarkers detection in blood such as amyloid ² peptide 42 (A²42) and amyloid ²peptide 40 (A²40). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARROTE, BEATRIZ L.; SANTOS, ADRIANO; BUENO, PAULO R.. Label-free capacitive assaying of biomarkers for molecular diagnostics. Nature Protocols, v. 15, n. 12, OCT 2020. (17/02974-3, 18/26273-7, 16/17185-1, 17/24839-0)

GARROTE, BEATRIZ LUCAS; LOPES, LAIS C.; PINZON, EDGAR F.; MENDONCA-NATIVIDADE, FLAVIA C.; MARTINS, RONALDO B.; SANTOS, ADRIANO; ARRUDA, EURICO; BUENO, PAULO R.. Reagentless Quantum-Rate-Based Electrochemical Signal of Graphene for Detecting SARS-CoV-2 Infection Using Nasal Swab Specimens. ACS SENSORS, v. 7, n. 9, p. 9-pg., 2022-09-01. (17/24839-0, 18/23577-5, 18/24525-9, 20/12854-8, 18/26273-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GARROTE, Beatriz Lucas. Label-Free electrochemical capacitive biological sensors for molecular diagnostics. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.