DNA metilation profile of mother-concept binomy in the context of Maternal Obesity and fetal and neonatal adiposity

• Grant number: 18/17824-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2019
• End date: November 30, 2021
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Patricia Helen de Carvalho Rondó
• Grantee: Perla Pizzi Argentato
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/03333-6 - The relationship between maternal adiposity and adiposity of the offspring in the fetal, neonatal and infant periods: a prospective population-based study, AP.TEM

Abstract

Obesity is a worldwide public health problem. It is known that the intrauterine environment can program the fetus to develop Obesity later in life. A recognized epigenetic mechanism by which the adverse effects of the intrauterine environment are transferred to the offspring is DNA methylation. However, the relationship between intrauterine exposures and changes in epigenetics, especially regarding fetal and newborn adiposity, is not clear in the literature. Objective: to evaluate the relationship between Maternal Obesity and maternal DNA methylation profile of the maternal and cord blood, and placenta tissues with fetal and neonatal adiposity. Methodology: a population of 12 pregnant women with previous Obesity, 12 with eutrophy and 12 eutrophic pregnant women with excess weight gain during pregnancy, according to recommendation of the Institute of Medicine (2009) and their respective concepts will be involved in this study. The population was selected from the cohort entitled "Relation between Obesity maternal and adiposity of the concept in the fetal, neonatal and first year of life: prospective population-based study". Maternal blood samples will be collected from the women in the third trimester of pregnancy and umbilical cord blood will be collected for analysis of the methylation by ARRAY with the Infinium MethylationEPIC BeadChip850 (HM850) (Illumina, CA, USA). Candidate genes will be selected for the validation of hypo- and hypermethylated regions by quantitative PCR (qPCR) in maternal and cord blood and placenta tissues (decidua and villus). Fetal adiposity will be measured in the second and third trimesters by ultrasonography using the Philips HD11XE ultrasound scanner (Philips, USA) and neonatal adiposity will be measured 12-72h after delivery by plethysmography with PEOD POD (Cosmed®, USA). Maternal nutrition will be evaluated by two 24-hour recall by the Software Nutrition Data System for Research (NDSR, Minnesota, USA). For statistics we will use the unpaired T-tests and two-way ANOVA. Multiple linear regression models will be used to study associations between the variables of interest controlling by confounding factors. Linear models of repeated measures will be used to detect differences in baseline and follow-up data. The significance level will be a pd 0.05. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAIARA NAIANA DEJANI; CAROLINA F. NICOLETTI; PERLA PIZZI ARGENTATO; LUDMILLA DA SILVA PEREIRA; AMANDA CORREIA SARAIVA; LETÍCIA MONTANHA DE ASSIS; PATRÍCIA YURY NAKANDAKARE; LÍVIA PATRICIA RODRIGUES BATISTA; LAÍSLA DE FRANÇA DA SILVA TELES; MARIA PAULA LEITÃO; et al. Maternal plasma transforming growth factor-β1 (TGF-β1) and newborn size: the Araraquara Cohort Study. Jornal de Pediatria, v. 99, n. 3, p. 284-288, 2023-06-16. (15/03333-6, 18/17824-0, 17/07143-2, 18/19638-9)

ARGENTATO, PERLA PIZZI; MARCHESI, JORGE AUGUSTO PETROLI; DEJANI, NAIARA NAIANA; NAKANDAKARE, PATRICIA YURY; TELES, LAISLA DE FRANCA DA SILVA; BATISTA, LIVIA PATRICIA RODRIGUES; LEITAO, MARIA PAULA CARVALHO; LUZIA, LIANIA ALVES; RAMOS, ESTER SILVEIRA; RONDO, PATRICIA HELEN. The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition. FRONTIERS IN NUTRITION, v. 10, p. 16-pg., 2023-09-21. (15/03333-6, 18/17824-0, 17/07143-2)

DEJANI, NAIARA NAIANA; NICOLETTI, CAROLINA F.; ARGENTATO, PERLA PIZZI; PEREIRA, LUDMILLA DA SILVA; SARAIVA, AMANDA CORREIA; DE ASSIS, LETICIA MONTANHA; NAKANDAKARE, PATRICIA YURY; BATISTA, LIVIA PATRICIA RODRIGUES; TELES, LAISLA DE FRANCA DA SILVA; LEITAO, MARIA PAULA; et al. Maternal plasma transforming growth factor-b1 (TGF-b1) and newborn size: the Araraquara Cohort Study. Jornal de Pediatria, v. 99, n. 3, p. 5-pg., 2023-05-01. (15/03333-6, 18/17824-0, 18/19638-9, 17/07143-2)

NAKANDAKARE, P.; NICOLETTI, C. F.; NORONHA, N. Y.; NONINO, C. B.; ARGENTATO, P. P.; DEJANI, N. N.; LUZIA, L. A.; ROGERO, M. M.; RONDO, P. H. C.. Associations between obesity-related gene expression in maternal and cord blood and newborn adiposity: findings from the Araraquara Cohort study. International Journal of Obesity, MAY 2021. (18/17824-0, 17/07143-2, 15/03333-6)

NAKANDAKARE, P.; NICOLETTI, C. F.; NORONHA, N. Y.; NONINO, C. B.; ARGENTATO, P. P.; DEJANI, N. N.; LUZIA, L. A.; ROGERO, M. M.; RONDO, P. H. C.. Associations between obesity-related gene expression in maternal and cord blood and newborn adiposity: findings from the Araraquara Cohort study. International Journal of Obesity, v. 45, n. 9, p. 1958-1966, SEP 2021. (18/17824-0, 17/07143-2, 15/03333-6)

PERLA PIZZI ARGENTATO; JOÃO VICTOR DA SILVA GUERRA; LIANIA ALVES LUZIA; ESTER SILVEIRA RAMOS; MARIANA MASCHIETTO; PATRÍCIA HELEN DE CARVALHO RONDÓ. Integrative network analysis of differentially methylated regions to study the impact of gestational weight gain on maternal metabolism and fetal-neonatal growth. GENETICS AND MOLECULAR BIOLOGY, v. 47, n. 1, 2024-03-25. (15/03333-6, 18/17824-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ARGENTATO, Perla Pizzi. DNA methylation profile of the mother-child binomial in maternal obesity, fetal and neonatal adiposity. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Saúde Pública (FSP/CIR) São Paulo.