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Molecular characterization of the genotypic diversity, resistance profile and pathogenic potential of Salmonella infantis strains isolated from multiple sources in Brazil

Grant number: 19/06947-6
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2019
Effective date (End): December 31, 2020
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Juliana Pfrimer Falcão
Grantee:Felipe Pinheiro Vilela
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):19/24815-0 - Whole-genome sequencing and phylogenetic analysis of Salmonella infantis strains isolated from multiple sources in Brazil, BE.EP.MS

Abstract

Salmonellosis caused by non-typhoidal serovars is among one of the most reported foodborne infection worldwide. Strains of Salmonella Infantis (S. Infantis), a non-specific host serovar, are capable to infect multiple animal reservoirs besides humans and have been reported presenting multidrug resistant profiles. This project aims to verify the genetic diversity of S. Infantis strains isolated from multiple sources in Brazil and also to determine the pathogenic potential and the phenotypic and genotypic antimicrobial resistance profiles to. For that, 149 S. Infantis strains isolated from humans, animals, food and the environment in Brazil between 2013 and 2018 will be molecularly typed by Pulsed-field gel electrophoresis (PFGE) and, based on the whole-genome sequencing (WGS) of these strains it will be typed by Single-Nucleotide Polymorphisms (SNPs), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and core genome Multilocus Sequencing Type (cgMLST). The presence of 16 virulence genes related to Salmonella pathogenicity islands (SPI-1 and SPI-2) will also be verified through data generated by WGS. Moreover, the antimicrobial susceptibility profile will be determined against 18 antimicrobial agents by the disk-diffusion test, as well as the presence of some related resistance genes and quinolone resistance determining region (QRDRs) mutations of all the strains will be searched. The results to be obtained should contribute to a better characterization of the circulating S. Infantis strains in Brazil in recent years, as well as elucidate possible characteristics and/or genotypic profiles to provide a better understanding of the infection and disease caused by this serovar of great clinical importance.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PAZIANI, MARIO HENRIQUE; BRAZ, VANIA SANTOS; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; VON ZESKA KRESS, MARCIA REGINA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil. INFECTION GENETICS AND EVOLUTION, v. 80, JUN 2020. Web of Science Citations: 0.
VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil. Brazilian Journal of Microbiology, NOV 2019. Web of Science Citations: 0.
VILELA, FELIPE PINHEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Analysis of Resistance Gene Prevalence in Whole-Genome Sequenced Enterobacteriales from Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 6 NOV 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.