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Analysis of adipogenesis related genes and intramuscular fat composition in Angus X Nellore cattle of different gender status

Grant number: 17/08075-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2019
Effective date (End): October 31, 2020
Field of knowledge:Agronomical Sciences - Animal Husbandry
Principal Investigator:Angélica Simone Cravo Pereira
Grantee:Lenise Freitas Mueller da Silveira
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The goal of this study is to analyze adipogenesis related genes and to evaluate the fatty acid profile of intramuscular fat in different meat cuts (Longissimus and Triceps brachii) of Angus x Nellore cattle from three gender status (bulls, steers, and heifers). Were used one hundred and fifty animals, from the Brazil Angus Beef Certified Program, slaughtered with an average age of 16 months, confined and assigned into three gender status. The experimental design considered was completely randomized, and the animals were kept under the same management conditions. All animals were fed ad libitum (86% concentrate), with an expectation of average daily gain of approximately 1.4 kg. At the end of the experimental period (150 days), the animals were harvested. During the slaughter, samples of blood from the animals were collected for hormonal quantification and small samples of two different cuts of meat were collected for extraction of RNA and proteins involved in adipogenesis. The pH of the carcass was measured 24 h after slaughter and during deboning were performing marbling score and the subcutaneous fat thickness in the Longissimus muscle, between the 12th and 13th ribs. A 1 cm thick sample of the same muscle were collected for analyses of total lipids content. Additionally, samples of the two different muscles mentioned above were collected, vacuum packed individually and frozen for analysis of the fatty acid profile. The statistical analyses will be performed by the proc MIXED SAS® (version 9.2), in split-plot design with mixed models, considering a 3x2 factorial arrangement. It is expected that the sexual condition influences the expression of genes involved in adipocyte formation and the fatty acid profile in different muscles. The results may suggest which cut would be healthier, as regards the profile of fatty acids for human health.