Encapsulation of Bacillus thuringiensis (Bacillales: Bacillaceae) in polymeric microparticles and association with nanoencapsulated chemical agent: evaluation of biological activity and mechanisms of action

• Grant number: 18/21142-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2019
• End date: November 01, 2022
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Ricardo Antonio Polanczyk
• Grantee: Jhones Luiz de Oliveira
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity, AP.TEM
• Associated scholarship(s): 20/12779-6 - Evaluation of the mechanisms of action of Bacillus thuringiensis (Bacillales: Bacillaceae) encapsulated in polymeric microparticles, BE.EP.PD

Abstract

New technologies must be adopted with the objective of optimizing productivity and adding development to Brazilian agribusiness. However, this development must be based on environmentally sustainable strategies. Microencapsulation has been shown to be an excellent alternative to protect biological control agents from external factors, increasing their effectiveness. In the case of chemical control agents, the use of nanostructured systems has important characteristics such as the modified release, reduction of the amount of active and reduction of the toxicity to non-target organisms. In this context, this proposal intends to produce a hybrid system composed of the mixture of different Bacillus thuringiensis strains encapsulated in polymeric microparticles in association with a nanoparticle release system for chemical control agents. As steps, compatibility tests will be carried out between chemical and biological control agents. Subsequently, the nanocarriers for the chemical ingredients will be prepared and characterized. The hybrid systems will be produced and characterized, being evaluated the biological activity as well as studies of mechanism of action. In this way, the development of products that associate micro and nanotechnology with the encapsulation of control agents can be achieved through the integration between basic and applied science, providing alternatives to integrated pest management in Agriculture. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALBUQUERQUE, FELICIA PEREIRA; DE OLIVEIRA, JHONES LUIZ; DOS SANTOS MACHADO, LEILA; RICHARDI, VINICIUS SOBRINHO; DA SILVA, MARIO ANTONIO NAVARRO; POMPEO, MARCELO LUIZ MARTINS; FRACETO, LEONARDO FERNANDES; CARLOS, VIVIANE MOSCHINI. Use of nontarget organism Chironomus sancticaroli to study the toxic effects of nanoatrazine. ECOTOXICOLOGY, v. 30, n. 4, p. 733-750, MAY 2021. (18/21142-1, 16/17266-1, 17/21004-5)

LUIS, ANGELICA I. S.; CAMPOS, ESTEFANIA V. R.; OLIVEIRA, JHONES L.; VALLIM, JOSE HENRIQUE; PROENCA, PATRICIA L. F.; CASTANHA, RODRIGO F.; DE CASTRO, VERA L. S. S.; FRACETO, LEONARDO F.. Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture. JOURNAL OF NANOBIOTECHNOLOGY, v. 19, n. 1, MAY 31 2021. (18/21142-1, 17/21004-5, 17/24402-1)

DE OLIVEIRA, JHONES L.; FRACETO, LEONARDO FERNANDES; BRAVO, ALEJANDRA; POLANCZYK, RICARDO ANTONIO. Encapsulation Strategies for Bacillus thuringiensis: From Now to the Future. Journal of Agricultural and Food Chemistry, v. 69, n. 16, p. 4564-4577, APR 28 2021. (20/12779-6, 18/21142-1, 17/21004-5)

GALHARDI, JULIANA APARECIDA; DE OLIVEIRA, JHONES LUIZ; GHOSHAL, SUBHASIS; FRACETO, LEONARDO FERNANDES. Soil Enzyme Responses to Polymeric Nanopesticides: An Ecological Risk Analysis Approach to Promote Sustainable Agriculture. ACS AGRICULTURAL SCIENCE & TECHNOLOGY, v. 2, n. 3, p. 10-pg., 2022-06-20. (17/21004-5, 18/21142-1)

CAMPOS, ESTEFANIA V. R.; PEREIRA, ANDERSON E. S.; DE OLIVEIRA, JHONES LUIZ; CARVALHO, LUCAS BRAGANCA; GUILGER-CASAGRANDE, MARIANA; DE LIMA, RENATA; FRACETO, LEONARDO FERNANDES. How can nanotechnology help to combat COVID-19? Opportunities and urgent need. JOURNAL OF NANOBIOTECHNOLOGY, v. 18, n. 1, SEP 5 2020. (17/21004-5, 19/05100-0, 18/21142-1, 18/23608-8, 20/05816-2, 17/24402-1)

ROGERIO, CAROLINA B.; ABRANTES, DANIELE CARVALHO; DE OLIVEIRA, JHONES L.; DE ARAUJO, DANIELE RIBEIRO; DA COSTA, TAIS GERMANO; DE LIMA, RENATA; FRACETO, LEONARDO FERNANDES. Cellulose Hydrogels Containing Geraniol and Icaridin Encapsulated in Zein Nanoparticles for Arbovirus Control. ACS APPLIED BIO MATERIALS, v. 5, n. 3, p. 11-pg., 2022-03-21. (20/05415-8, 18/02404-5, 18/21142-1)

MUJTABA, MUHAMMAD; WANG, DEPENG; CARVALHO, LUCAS BRAGANCA; OLIVEIRA, JHONES LUIZ; SANTO PEREIRA, ANDERSON DO ESPIRITO; SHARIF, RAHAT; JOGAIAH, SUDISHA; PAIDI, MURALI KRISHNA; WANG, LICHEN; ALI, QASID; et al. Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions. ACS AGRICULTURAL SCIENCE & TECHNOLOGY, v. 1, n. 5, p. 19-pg., 2021-10-18. (17/21004-5, 18/21142-1, 18/23608-8)

CAMARA, MARCELA CANDIDO; MONTEIRO, RENATA APARECIDA; CARVALHO, LUCAS BRAGANCA; OLIVEIRA, JHONES LUIZ; FRACETO, LEONARDO F.. Enzyme Stimuli-Responsive Nanoparticles for Bioinsecticides: An Emerging Approach for Uses in Crop Protection. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 9, n. 1, p. 106-112, JAN 11 2021. (18/23608-8, 18/21142-1)

DE ALBUQUERQUE, FELICIA PEREIRA; DE OLIVEIRA, JHONES LUIZ; MOSCHINI-CARLOS, VIVIANE; FRACETO, LEONARDO FERNANDES. An overview of the potential impacts of atrazine in aquatic environments: Perspectives for tailored solutions based on nanotechnology. Science of The Total Environment, v. 700, JAN 15 2020. (18/21142-1, 17/21004-5)

PEREIRA, ANDERSON DO ESPIRITO SANTO; DE OLIVEIRA, JHONES LUIZ; SAVASSA, SUSILAINE MAIRA; ROGERIO, CAROLINA BARBARA; DE MEDEIROS, GERSON ARAUJO; FRACETO, LEONARDO FERNANDES. Lignin nanoparticles: New insights for a sustainable agriculture. JOURNAL OF CLEANER PRODUCTION, v. 345, p. 16-pg., 2022-04-15. (17/21004-5, 18/21142-1, 20/12779-6, 20/05415-8)

MONTEIRO, RENATA APARECIDA; CAMARA, MARCELA CANDIDO; DE OLIVEIRA, JHONES LUIZ; RAMOS CAMPOS, ESTEFANIA VANGELIE; CARVALHO, LUCAS BRAGANCA; DE FREITAS PROENCA, PATRICIA LUIZA; GUILGER-CASAGRANDE, MARIANA; LIMA, RENATA; DO NASCIMENTO, JOACIR; GONCALVES, KELLY CRISTINA; et al. Zein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture. JOURNAL OF HAZARDOUS MATERIALS, v. 417, SEP 5 2021. (17/21004-5, 17/13328-5, 18/21142-1, 18/23608-8, 20/05816-2)

MEIRELLES, LAURA NOBREGA; MESQUITA, EMILY; CORREA, THAIS ALMEIDA; BITENCOURT, RICARDO DE OLIVEIRA BARBOSA; OLIVEIRA, JHONES LUIZ; FRACETO, LEONARDO FERNANDES; CAMARGO, MARIANA GUEDES; BITTENCOURT, VANIA RITA ELIAS PINHEIRO. Encapsulation of entomopathogenic fungal conidia: evaluation of stability and control potential of Rhipicephalus microplus. TICKS AND TICK-BORNE DISEASES, v. 14, n. 4, p. 10-pg., 2023-04-25. (18/21142-1, 17/21004-5)

BOFF, JESSICA S.; REIS, ALEXANDRE C.; DE OLIVEIRA, JHONES L.; GROSS, RENATA B.; FRACETO, LEONARDO F.; MELO, ADRIANO A.; BERNARDI, ODERLEI. Development and biological evaluation of nanoencapsulated-based pyrethroids with synergists for resistance management of two soybean pests: insights for new insecticide formulations. Pest Management Science, v. 79, n. 3, p. 9-pg., 2022-11-29. (17/21004-5, 18/21142-1)