Genome-wide approaches to reveal epigenomic structure in trypanosomes subjected to metabolic states changes and during cell cycle

• Grant number: 19/19690-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2019
• End date: August 13, 2021
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Agreement: MRC, UKRI ; Newton Fund, with FAPESP as a partner institution in Brazil
• Principal Investigator: Ariel Mariano Silber
• Grantee: Alex Ranieri Jerônimo Lima
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/14432-3 - A network for an integrative biology in neglected diseases: bridging epigenetics, metabolism and cell cycle in pathogenic trypanosomatids, AP.TEM

Abstract

Parasites submitted to different metabolic backgrounds, such as severe metabolic stress or metabolic stress conditions in the presence of single carbon and energy sources for maintenance (PBS supplemented with glucose, proline, histidine or leucine) could have different epigenomes. These differences could affect cell cycle, differentiation or host-cell invasion among other processes. In this proposal, we will evaluate the epigenome of parasites maintained under different metabolic conditions. We will take advantage of two genome-wide methods that allow the identification of nucleosomes position as well as putative regulatory sequences, possibly associated with highly active transcribed regions. These two approaches will be complementary and will make it possible to discriminate unbiased important genomic regions associated either with metabolic states or cell cycle.To this end, an intense bioinformatic approach to integrate these datasets as well as to explore functional annotation available at public datasets will also be implemented. In addition, comparison between T.cruzi and Leishmania data will be used as a valuable resource to better understand similarities and differences inherent to their biology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, ALEX R. J.; DE ARAUJO, CHRISTIANE B.; BISPO, SALOE; PATANE, JOSE; SILBER, ARIEL M.; ELIAS, M. CAROLINA; DA CUNHA, JULIA P. C.. Nucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life forms. PLOS PATHOGENS, v. 17, n. 1, JAN 2021. (18/15553-9, 19/19690-3, 18/14432-3, 16/50050-2, 13/07467-1)

JERONIMO LIMA, ALEX RANIERI; DE SOUSA SILVA, HERBERT GUIMARAES; POUBEL, SALOE; ROSON, JULIANA NUNES; OLIVEIRA DE LIMA, LOYZE PAOLA; COSTA-SILVA, HELLIDA MARINA; GONCALVES, CAMILA SILVA; GALANTE, PEDRO A. F.; HOLETZ, FABIOLA; MOTTA, MARIA CRISTINA MACHADO M.; et al. Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci. EPIGENETICS & CHROMATIN, v. 15, n. 1, p. 16-pg., 2022-06-01. (13/07467-1, 18/15553-9, 20/02708-4, 18/14432-3, 19/19834-5, 19/19690-3)