Chasing for chromatine-associated proteins and histone PTMs patterns associated to cell stages and metabolic states in trypanosomes

• Grant number: 19/21354-1
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): January 01, 2020
• Effective date (End): December 31, 2021
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Cooperation agreement: MRC, UKRI ; Newton Fund, with FAPESP as a partner institution in Brazil
• Principal researcher: Ariel Mariano Silber
• Grantee: Ana Paula de Jesus Menezes
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/14432-3 - A network for an integrative biology in neglected diseases: bridging epigenetics, metabolism and cell cycle in pathogenic trypanosomatids, AP.TEM

Abstract

Parasites submitted to different metabolic backgrounds, such as severe metabolic stress or metabolic stress conditions in the presence of single carbon and energy sources for maintenance (PBS supplemented with glucose, proline, histidine or leucine) could have different epigenomes. These differences could affect cell cycle, differentiation or host-cell invasion among other processes. In this proposal, we will evaluate the epigenome of parasites maintained under different metabolic conditions. Using a cutting-edge pipeline for histone PTM analysis we expect to obtain a large dataset of quantitative measurements in the above mentioned scenarios. The PTMs at the chromatin associated proteins will also be obtained. After an intense data analysis, which includes integration of both proteomics datasets generated, it will be possible to identify key epigenetic markers associated either with metabolic states or cell cycle. Then, we will choose targets to better explore their putative role. Finally, target biological function will be explored by evaluating: genomic location, cellular location and the expression dynamics. (AU)