Development of open tubular capillary columns for micro and nano liquid chromatography coupled to sequential mass spectroscopy

• Grant number: 19/26263-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: February 01, 2020
• End date: April 09, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Fernando Mauro Lanças
• Grantee: Natalia Gabrielly Pereira dos Santos
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications, AP.TEM

Abstract

Liquid Chromatography (LC) is one of the most versatile separation techniques and is employed in a number of relevant areas of scientific knowledge. Its miniaturization, understood here as the reduction of the internal diameter of the columns, followed the introduction of capillary columns in gas chromatography by Golay. Therefore, the current interest is focused on the development of new LC columns, aiming at increasing the efficiency of the technique and implementing the many benefits inherent to it. These mainly include system miniaturization and its implications such as solvent and sample economy and coupling with detection systems commonly used in gas chromatography such as mass spectrometry. Among the columns employed in LC, we highlight the open tubular capillary columns of the type PLOT (Porous Layer Open Tubular) and WCOT (Wall Coated Open Tubular), with few studies on the evaluation and use of new materials and techniques in its preparation. In this context, this research project aims to study new materials for both types of columns, together with an optimization of the column preparation system to be operated in line with miniaturized systems (¼-LC and nano-LC) coupled to sequential mass spectrometry (MS/MS). (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, NATALIA GABRIELLY; MEDINA, DEYBER ARLEY VARGAS; LANCAS, FERNANDO MAURO. Microextraction by packed sorbent of N-nitrosamines from Losartan tablets using a high-throughput robot platform followed by liquid chromatography-tandem mass spectrometry. JOURNAL OF SEPARATION SCIENCE, v. 46, n. 17, p. 13-pg., 2023-07-03. (15/15462-5, 19/22724-7, 17/02147-0, 14/07347-9, 19/26263-4)

DOS SANTOS, NATALIA GABRIELLY PEREIRA; MACIEL, EDVALDO VASCONCELOS SOARES; MEDINA, DEYBER ARLEY VARGAS; LANCAS, FERNANDO MAURO. NanoLC-EI-MS: Perspectives in Biochemical Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 14, p. 14-pg., 2023-07-01. (19/22724-7, 17/21984-0, 17/02147-0, 17/21985-6, 19/26263-4)

SOARES MACIEL, EDVALDO VASCONCELOS; PEREIRA DOS SANTOS, NATALIA GABRIELLY; VARGAS MEDINA, DEYBER ARLEY; LANCAS, FERNANDO MAURO. Electron ionization mass spectrometry: Quo vadis?. ELECTROPHORESIS, v. 43, n. 15, p. 14-pg., 2022-05-20. (19/26263-4, 19/22724-7, 17/02147-0, 14/03765-0, 15/15462-5, 14/07347-9)

VARGAS MEDINA, DEYBER ARLEY; PEREIRA DOS SANTOS, NATALIA GABRIELLY; DA SILVA BURATO, JULIANA SOARES; BASOLLI BORSATTO, JOAO VICTOR; LANCAS, FERNANDO MAURO. An overview of open tubular liquid chromatography with a focus on the coupling with mass spectrometry for the analysis of small molecules. Journal of Chromatography A, v. 1641, MAR 29 2021. (19/26263-4, 19/22724-7, 14/07347-9, 17/02147-0, 14/03765-0, 15/15462-5)

VARGAS MEDINA, DEYBER ARLEY; PEREIRA DOS SANTOS, NATALIA GABRIELLY; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Current prospects on nano liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS). Journal of Liquid Chromatography & Related Technologies, v. 44, n. 17-20, p. 10-pg., 2022-08-17. (19/26263-4, 19/22724-7, 17/02147-0, 14/03765-0, 15/15462-5, 14/07347-9)

SANTOS, NATALIA G. P.; MEDINA, DEYBER A. V.; LANCAS, FERNANDO M.. Development of Wall-Coated Open Tubular Columns and Their Application to Nano Liquid Chromatography Coupled to Tandem Mass Spectrometry. Molecules, v. 28, n. 13, p. 15-pg., 2023-07-01. (15/15462-5, 19/22724-7, 17/02147-0, 14/07347-9, 19/26263-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Natalia Gabrielly Pereira dos. Development of open tubular columns for application in nano liquid chromatography coupled to tandem mass spectrometry. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.