Role of interleukin-6 in the responses of the autophagic pathway in the heart of mice submitted in acute exhaustive physical exercise

• Grant number: 19/17058-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2020
• End date: February 28, 2022
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Adelino Sanchez Ramos da Silva
• Grantee: Rafael Lemes Rovina
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Our research group verified that the imbalance between the excess of physical exercise and the adequate period of rest is linked with signals of pathological hypertrophy cardiac, lower phosphorylation of protein AMP-activated protein kinase (AMPK) in tyrosine 172, and higher concentrations of interleukin-6 (IL-6) in serum and cardiac tissue in mice. As known, which the increase in IL-6 is capable of inhibition the process of autophagy, whose activation is considered a promising therapeutic target for the prevention and treatment of cardiovascular diseases. In this sense, the main objective of this research project will be to investigate the role of IL-6 in the responses of the autophagic pathway in the heart of mice submitted in acute exhaustive physical exercise. For this, mice wild-type C57BL/6J (WT) and knockout IL-6 (KO) will be submitted to a protocol of acute exhaustive physical exercise. Serum samples and cardiac muscle will be collected immediately, 1 hour and 3 hours after the exhaustive exercise protocol. The functional parameters, serums and molecular will be analyzed by the echocardiogram, autophagic flux, further the techniques of Real-time-RT-PCR (Reverse Transcription Polymerase Chain Reaction) and immunoblotting. In addition, according to the statistical distribution of the data, parametric or non-parametric tests will be used to analyze the results. The significance level of p <0.05 will be adopted. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, ANA P.; DA ROCHA, ALISSON L.; TEIXEIRA, GIOVANA R.; ROVINA, RAFAEL L.; VERAS, ALLICE S. C.; FRANTZ, FABIANI; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; et al. Rapamycin did not prevent the excessive exercise-induced hepatic fat accumulation. Life Sciences, v. 306, p. 12-pg., 2022-07-15. (17/12765-2, 19/17058-8, 17/09038-1, 19/00137-2, 19/15428-2, 17/19869-8)

ROVINA, RAFAEL L.; PINTO, ANA P.; MUNOZ, VITOR R.; DA ROCHA, ALISSON L.; REBELO, MACARIO A.; TEIXEIRA, GIOVANA R.; TAVARES, MARIA EDUARDA A.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; et al. Genetic deletion of IL-6 increases CK-MB, a classic cardiac damage marker, and decreases UPRmt genes after exhaustive exercise. Cell Biochemistry and Function, v. 40, n. 4, p. 10-pg., 2022-04-12. (17/19869-8, 17/09038-1, 19/17058-8, 17/12765-2)

DA ROCHA, ALISSON L.; ROVINA, RAFAEL L.; PINTO, ANA P.; MARAFON, BRUNO B.; DA SILVA, LILIAN E. C. M.; SIMABUCO, FERNANDO M.; FRANTZ, FABIANI G.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; et al. Interleukin-6 ablation does not alter morphofunctional heart characteristics but modulates physiological and inflammatory markers after strenuous exercise. CYTOKINE, v. 142, p. 11-pg., 2021-03-23. (19/17058-8, 17/09038-1, 17/17646-1, 19/00855-2)

DA ROCHA, ALISSON L.; ROVINA, RAFAEL L.; PINTO, ANA P.; MARAFON, BRUNO B.; DA SILVA, LILIAN E. C. M.; SIMABUCO, FERNANDO M.; FRANTZ, FABIANI G.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; et al. nterleukin-6 ablation does not alter morphofunctional heart characteristics but modulates physiological and inflammatory markers after strenuous exercis. CYTOKINE, v. 142, JUN 2021. (19/17058-8, 17/17646-1, 17/09038-1, 19/00855-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROVINA, Rafael Lemes. Role of interleukin-6 in the responses of the autophagy pathway in the heart of mice submitted to exhaustive acute physical exercise. 2022. Master's Dissertation - Universidade de São Paulo (USP). Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP) Ribeirão Preto.