Study on the release of caffeine from magnetic xanthan scaffolds under magnetic stimulus

• Grant number: 20/03248-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2020
• End date: August 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Denise Freitas Siqueira Petri
• Grantee: Vitoria Hashimoto Lima
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine, AP.TEM

Abstract

In this project, xanthan based scaffolds containing magnetic nanoparticles (MNP) will be used as reservoirs for bioactive molecules. First, MNP will be synthesized ex situ and then they will be incorporated in the scaffolds by immersion. Caffeine, 1,3,7-trimethylxanthine, belonging to the family of purines will be tested. The amount of incorporated caffeine will be optimized in order to keep cell viability at high leve. The caffeine release from the magnetic scaffolds will be tested as a function of pH, ionic strength, absence and presence of external magnetic field (magnets). The release conditions will be optimized in order to be applied in the proliferation and differentiation of neuronal cells , in collaboration with Prof. Henning Ulrich.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALAVARSE, ALEX CARVALHO; FRACHINI, EMILLI CAROLINE GARCIA; DA SILVA, RAFAEL LEONARDO CRUZ GOMES; LIMA, VITORIA HASHIMOTO; SHAVANDI, AMIN; PETRI, DENISE FREITAS SIQUEIRA. Crosslinkers for polysaccharides and proteins: Synthesis conditions, mechanisms, and crosslinking efficiency, a review. International Journal of Biological Macromolecules, v. 202, p. 39-pg., 2022-01-25. (20/03248-7, 20/01907-3, 19/22671-0, 20/05632-9, 18/13492-2)