Diversity of piroplasmids in wild mammals and birds from Brazil

• Grant number: 20/07826-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2021
• End date: June 01, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcos Rogério André
• Grantee: Ana Cláudia Calchi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 22/16555-0 - Standardization of a digital PCR for the detection of piroplasmida DNA in wild animals' biological samples from Brazil, BE.EP.DR

Abstract

Piroplasmids are tick-borne apicomplexan protozoans that include the genera Babesia, Rangelia, Cytauxzoon and Theileria. There is a wide diversity of species that affect different taxons of mammals and birds and can cause diseases in animals and humans. Studies aimed at detecting piroplasmids in the Brazilian fauna, which is extremely diverse, show that different and new species of these protozoans circulate in the country. This study aims to investigate the occurrence and the genetic diversity of piroplasmids in different taxonomic groups of vertebrates in Brazil. For this purpose, DNA aliquots will be extracted from 1,557 biological samples of free living animals belonging to seven taxonomic groups (311 Pilosa, 20 Cingulata, 367 Chiroptera, 188 Artiodactyla, 26 Carnivora, 129 Rodentia, eight Didelphimorphia and 500 Passeriformes) from different States of the Country (SP, MT, MS, PA, RO, AC, PR). The biological samples will be subjected to real-time and conventional PCR assays based on 18S rRNA, hsp-70, cox-1, cox-3, cytB, beta-tubulin, in the intergenic regions ITS-1 and ITS-2 and in the lsu5-lsu4 piroplasmid region. Positive samples will be purified and sequenced by the Sanger method. Amplicons showing multiple peaked samples in the chromatograms will be submitted to gene cloning. The phylogenetic positioning of the obtained sequences will be performed by dendograms. Finally, the sequence alignment will be used to calculate the nucleotide diversity and the level of polymorphisms for the analysis of the genetic diversity of agents. The genealogy of nucleotide sequences will be performed using the Splitstree program. This study will contribute to the study of piroplasmid diversity among Brazilian wildlife. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALCHI, ANA CLAUDIA; BRAGA, LAIZA DE QUEIROZ VIANA; BASSINI-SILVA, RICARDO; CASTRO-SANTIAGO, ANACAROLINA; HERRERA, HEITOR MIRAGLIA; SOARES, JOAO FABIO; BARROS-BATTESTI, DARCI MORAES; MACHADO, ROSANGELA ZACARIAS; ROCHA, FABIANA LOPES; ANDRE, MARCOS ROGERIO. Phylogenetic inferences based on distinct molecular markers reveals a novel Babesia ( Babesia pantanalensis nov. sp.) and a Hepatozoon americanum-related genotype in crab-eating foxes ( Cerdocyon thous ). Experimental Parasitology, v. 262, p. 10-pg., 2024-05-22. (20/11755-6, 20/12037-0, 20/07826-5, 21/06758-9, 22/08543-2)

CALCHI, ANA CLAUDIA; DUARTE, JOSE MAURICIO BARBANTI; CASTRO-SANTIAGO, ANA CAROLINA; BASSINI-SILVA, RICARDO; BARROS-BATTESTI, DARCI MORAES; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Genetic diversity of Theileria spp. in deer (Artiodactyla: Cervidae) from Brazil. Parasitology Research, v. 123, n. 11, p. 17-pg., 2024-11-01. (20/11755-6, 20/12037-0, 20/07826-5, 21/06758-9, 22/08543-2)

BARROS-BATTESTI, DARCI MORAES; ANDRE, MARCOS ROGERIO; HOPPE, ESTEVAM GUILHERME LUX; SERON, GUSTAVO; BASSINI-SILVA, RICARDO; CALCHI, ANA CLAUDIA; ANDRADE, LIVIA OLIVEIRA; PERIN, PATRICIA PARREIRA; MARTINS, THIAGO FERNANDES; CASTRO-SANTIAGO, ANA CAROLINA; et al. Noteworthy records of the ticks Ornithodoros rostratus and Amblyomma sculptum parasitizing Pteronura brasiliensis in the central-western region of Brazil, with pathogen investigation notes. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 33, n. 1, p. 7-pg., 2024-01-01. (20/11755-6, 18/24667-8, 20/05987-1, 20/07826-5, 19/19853-0, 19/03167-0, 17/01416-7, 21/06758-9)

ANDRE, MARCOS ROGERIO; CALCHI, ANA CLAUDIA; FURQUIM, MARIA EDUARDA CHIARADIA; DE ANDRADE, ISABELA; ARANTES, PAULO VITOR CADINA; DE MELO LOPES, LARA CRISTINA; DEMARCHI, IURI KAUAN LINS DO NASCIMENTO; FIGUEIREDO, MAYRA ARAGUAIA PEREIRA; DE PAULA LIMA, CIRILO ANTONIO; MACHADO, ROSANGELA ZACARIAS. Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil. PATHOGENS, v. 11, n. 1, JAN 2022. (20/12773-8, 19/10101-5, 19/19027-2, 18/02753-0, 19/26109-5, 20/07826-5, 20/12037-0)

KRAWCZAK, FELIPE DA SILVA; CALCHI, ANA CLAUDIA; NEVES, LUCIANNE CARDOSO; DIAS, SARAH ALVES; DA SILVA, BIANCA BARBARA FONSECA; PAULA, WARLEY VIEIRA DE FREITAS; DE PAULA, LUIZA GABRIELLA FERREIRA; TAVARES, MARIANA AVELAR; PADUA, GRACIELLE TELES; DE LIMA, NICOLAS JALOWITZKI; et al. Phylogenetic Inferences Based on Distinct Molecular Markers Confirm a Novel Babesia Species (Babesia goianiaensis nov. sp.) in Capybaras (Hydrochoerus hydrochaeris) and Associated Ticks. MICROORGANISMS, v. 11, n. 8, p. 13-pg., 2023-08-01. (20/07826-5, 20/12037-0)

DARCI MORAES BARROS-BATTESTI; MARCOS ROGÉRIO ANDRÉ; ESTEVAM GUILHERME LUX HOPPE; GUSTAVO SERON SANCHES; RICARDO BASSINI-SILVA; ANA CLAÚDIA CALCHI; LÍVIA OLIVEIRA ANDRADE; PATRÍCIA PARREIRA PERIN; THIAGO FERNANDES MARTINS; ANA CAROLINA CASTRO-SANTIAGO; et al. Interessantes registros dos carrapatos Ornithodoros rostratus e Amblyomma sculptum parasitando Pteronura brasiliensis na região centro-oeste do Brasil, com informações sobre a investigação de patógenos. Revista Brasileira de Parasitologia Veterinária, v. 33, n. 1, 2023-12-22. (21/06758-9, 20/05987-1, 17/01416-7, 19/03167-0, 20/11755-6, 19/19853-0, 20/07826-5, 18/24667-8)

BASSINI-SILVA, RICARDO; DAS CHAGAS, MARIA EDUARDA MORAES; MELLO-OLIVEIRA, VICTOR DE SOUZA; CALCHI, ANA CLAUDIA; CASTRO-SANTIAGO, ANA CAROLINA; ANDRADE, LIVIA DE OLIVEIRA; BENEDET, GABRIELA COELHO; PEREIRA, FERNANDA MARA ARAGAO MACEDO; SOARES-NETO, LAURO LEITE; HIPPOLITO, ALICIA GIOLO; et al. Eutrichophilus cordiceps Mjoberg, 1910 (Ischnocera: Trichodectidae) in Spiny Tree Porcupines (Coendou villosus): New locality records and the first molecular evidence of association with Bartonella sp. Parasitology International, v. 100, p. 7-pg., 2024-06-01. (18/24667-8, 20/07826-5, 19/19853-0, 23/06878-0, 23/09336-3, 17/01416-7, 21/06758-9)

COSTA OLIVEIRA BRAGA, MARIA DO SOCORRO; COSTA, FRANCISCO BORGES; CALCHI, ANA CLAUDIA; DE MELLO, VICTORIA VALENTE CALIFRE; MONGRUEL, ANNA CLAUDIA BAUMEL; DIAS, CLARA MORATO; BASSINI-SILVA, RICARDO; SILVA, ELLAINY MARIA CONCEICAO; PEREIRA, JOSE GOMES; RIBEIRO, LARISSA SARMENTO DOS SANTOS; et al. Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil. Acta Tropica, v. 244, p. 14-pg., 2023-05-30. (20/07826-5, 20/12037-0, 20/11755-6)

SANTANA, MATHEUS DE SOUZA; HOPPE, ESTEVAM GUILHERME LUX; CARRARO, PAULO EDUARDO; CALCHI, ANA CLAUDIA; DE OLIVEIRA, LARYSSA BORGES; DO AMARAL, RENAN BRESSIANINI; MONGRUEL, ANNA CLAUDIA BAUMEL; MACHADO, DALIA MONIQUE RIBEIRO; BURGER, KARINA PAES; BARROS-BATESTTI, DARCI MORAES; et al. Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas. TRANSBOUNDARY AND EMERGING DISEASES, v. 69, n. 5, p. 24-pg., 2022-07-11. (19/26403-0, 20/12037-0, 19/24726-7, 20/07826-5, 18/02753-0)

CALCHI, ANA CLAUDIA; YOGUI, DEBORA REGINA; ALVES, MARIO HENRIQUE; JEAN DESBIEZ, ARNAUD LEONARD; KLUYBER, DANILO; VULTAO, JULIANA GABOARDI; CADINA ARANTES, PAULO VITOR; DE SANTI, MARIELE; WERTHER, KARIN; GERALDES TEIXEIRA, MARTA MARIA; et al. Molecular detection of piroplasmids in mammals from the Superorder Xenarthra in Brazil. Parasitology Research, v. 122, n. 12, p. 12-pg., 2023-10-18. (21/00353-7, 20/12037-0, 20/07826-5)

BASSINI-SILVA, RICARDO; CASTRO-SANTIAGO, ANA CAROLINA; CALCHI, ANA CLAUDIA; PERLES, LIVIA; TAKATSU, JULIA CARVALHO; ALENCAR, ISABEL D. C. C.; OCHOA, RONALD; DOWLING, ASHLEY P. G.; WERTHER, KARIN; ANDRE, MARCOS ROGERIO; et al. Sleeping with the enemy: case reports of Ornithonyssus bursa (Berlese, 1888) (Mesostigmata: Macronyssidae) causing human dermatitis in Brazil. Parasitology Research, v. 121, n. 9, p. 9-pg., 2022-07-08. (21/06758-9, 18/24667-8, 19/15150-4, 21/02295-4, 20/07826-5, 20/11755-6, 19/19853-0, 17/01416-7)

MELLO-OLIVEIRA, VICTOR DE SOUZA; JACINAVICIUS, FERNANDO DE CASTRO; GUERRO-DE-SOUZA, MARIANE; DORNELAS JR, LEORMANDO FORTUNATO; CASTRO-SANTIAGO, ANA CAROLINA; CALCHI, ANA CLAUDIA; LOURENCO, ELIZABETE CAPTIVO; DOWLING, ASHLEY P. G.; FAMADAS, KATIA MARIA; ANDRE, MARCOS ROGERIO; et al. Contribution to the knowledge of Parichoronyssus bakeri Morales-Malacara and Guerrero, 2007 (Mesostigmata: Macronyssidae): new locality and host-association records with additional molecular data. ACAROLOGIA, v. 64, n. 1, p. 7-pg., 2023-12-14. (20/11755-6, 18/24667-8, 23/06748-9, 20/07826-5, 19/19853-0, 23/06878-0, 22/08620-7, 17/01416-7, 21/06758-9)

BASSINI-SILVA, RICARDO; CALCHI, ANA CLAUDIA; CASTRO-SANTIAGO, ANA CAROLINA; SICONELLI, MARCIO JUNIO LIMA; ANDRE, MARCOS ROGERIO; BARROS-BATTESTI, DARCI MORAES; JACINAVICIUS, FERNANDO DE CASTRO. Co-infestation caused by Goniodes pavonis (L., 1758) (Ischnocera: Goniodidae) and Goniocotes rectangulatus Nitzsch [In Giebel], 1866 on white Indian peafowl, Pavo cristatus L., 1758 (Galliformes: Phasianidaede) in Brazil. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 47, p. 4-pg., 2023-11-21. (20/11755-6, 18/24667-8, 20/07826-5, 19/19853-0, 17/01416-7, 21/06758-9)