In situ and operando studies of metaloenzymes for energy conversion and fuel bioelectrosynthesis

• Grant number: 20/04796-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2021
• End date: November 21, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Frank Nelson Crespilho
• Grantee: Graziela Cristina Sedenho
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 21/10307-2 - Engineering enzyme-displayed microbial cell surfaces and electrode surfaces for energy conversion and bioelectrosynthesis, BE.EP.PD

Abstract

Some subjects which have not yet been studied or not clarified in the bioelectrochemistry field have an immediate impact on the development and use of energy bioconversion and fuel bioelectrosynthesis technologies. They include: halide inhibition of the multi-copper oxidases enzymatic activity towards the molecular oxygen reduction reaction and the mechanism of the reverse reaction (H2O oxidation) catalyzed by these enzymes. Therefore, this project aims to answer such questions using electrochemical techniques coupled with spectroscopic techniques (FTIR, Raman and X-ray absorption spectroscopy), since they provide electrochemical control over the system by modulating enzymatic activity, while structural and electronic changes of the active sites are monitored. Understanding the aforementioned issues has an immediate impact on the development of more robust biocathodes for biofuel cells, in obtaining effective bioanodes in water splitting systems for H2 generation and can contribute to the conception of three-dimensional interactions existing in the enzymatic microenvironment for the development of effective biomimetic catalysts. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALI, MIAN A.; SEDENHO, GRAZIELA C.; PACHECO, JESSICA C.; IOST, RODRIGO M.; RAHMAN, ASMA; HASSAN, AYAZ; CARDOSO, DANIEL R.; GOMES, ROBERTO S.; CRESPILHO, FRANK N.. Metal-free, low-cost, and high-performance membraneless ethanol fuel cell. Journal of Power Sources, v. 551, p. 9-pg., 2022-10-08. (20/15098-0, 18/24485-7, 20/04796-8, 18/22214-6, 19/12053-8, 19/15333-1)

SEDENHO, GRAZIELA C.; COLOMBO, RAFAEL N. P.; CRESPILHO, FRANK N.. Insights from Enzymatic Catalysis: A Path towards Bioinspired High-Performance Electrocatalysts. CHEMCATCHEM, v. N/A, p. 18-pg., 2023-07-06. (21/05665-7, 22/09164-5, 18/22214-6, 20/04796-8)

COLOMBO, RAFAEL N. P.; SEDENHO, GRAZIELA C.; CRESPILHO, FRANK N.. Challenges in Biomaterials Science for Electrochemical Biosensing and Bioenergy. CHEMISTRY OF MATERIALS, v. 34, n. 23, p. 12-pg., 2022-10-17. (21/05665-7, 20/04796-8, 18/22214-6)

GHIRALDELLI, GUILHERME H. S.; IOST, RODRIGO M.; SEDENHO, GRAZIELA C.; COLOMBO, RAFAEL N. P.; CRESPILHO, FRANK N.. Yeast biofilm synapse: an intra-kingdom pathway to high-density current output in bioelectronic devices. JOURNAL OF MATERIALS CHEMISTRY B, v. N/A, p. 10-pg., 2025-03-11. (20/04796-8, 19/15333-1, 23/10667-4, 21/05665-7, 18/22214-6, 23/01529-7, 23/06283-6, 19/12053-8)

SEDENHO, GRAZIELA C.; NASCIMENTO, STEFFANE Q.; ZAMANI, MARJON; CRESPILHO, FRANK N.; FURST, ARIEL L.. Secondary Structure in Enzyme-Inspired Polymer Catalysts Impacts Water Oxidation Efficiency. ADVANCED SCIENCE, v. 11, n. 25, p. 6-pg., 2024-04-17. (18/22214-6, 20/04796-8, 21/10307-2)

BERTAGLIA, THIAGO; KERR, EMILY F.; SEDENHO, GRAZIELA C.; WONG, ANDREW A.; COLOMBO, RAFAEL N. P.; MACEDO, LUCYANO J. A.; IOST, RODRIGO M.; FARIA, LUANA C. I.; LIMA, FILIPE C. D. A.; TEOBALDO, GABRIEL B. M.; et al. Self-Gelling Quinone-Based Wearable Microbattery. ADVANCED MATERIALS TECHNOLOGIES, v. 9, n. 17, p. 11-pg., 2024-06-09. (22/09164-5, 20/12404-2, 16/24531-3, 19/21089-6, 21/05665-7, 19/15333-1, 20/04796-8, 17/20493-2, 20/03681-2, 18/22214-6, 23/01529-7, 23/17506-6)

MATTIOLI, ISABELA A.; CASTRO, KARLA R.; MACEDO, LUCYANO J. A.; SEDENHO, GRAZIELA C.; OLIVEIRA, MONA N.; TODESCHINI, IRIS; VITALE, PHELIPE M.; FERREIRA, SUZETE CLEUSA; MANULI, ERIKA R.; PEREIRA, GEOVANA M.; et al. Graphene-based hybrid electrical-electrochemical point-of-care device for serologic COVID-19 diagnosis. BIOSENSORS & BIOELECTRONICS, v. 199, p. 10-pg., 2022-03-01. (17/20493-2, 20/04796-8, 18/11071-0, 18/22214-6, 19/12053-8)

MATTIOLI, ISABELA A.; CASTRO, KARLA R.; SEDENHO, GRAZIELA C.; MACEDO, LUCYANO J. A.; OLIVEIRA, MONA N.; MANULI, ERIKA R.; SABINO, ESTER C.; CRESPILHO, FRANK N.. Expanding the application of graphene vertical devices to dual femtomolar detection of SARS-CoV-2 receptor binding domain in serum and saliva. BIOSENSORS & BIOELECTRONICS, v. 239, p. 6-pg., 2023-11-01. (20/04796-8, 17/20493-2, 19/12053-8, 18/11071-0, 19/15333-1, 18/22214-6)

SEDENHO, GRAZIELA C.; NECKEL, ITAMAR T.; COLOMBO, RAFAEL N. P.; PACHECO, JESSICA C.; BERTAGLIA, THIAGO; CRESPILHO, FRANK N.. Investigation of Water Splitting Reaction by a Multicopper Oxidase through X-ray Absorption Nanospectroelectrochemistry. ADVANCED ENERGY MATERIALS, v. 12, n. 47, p. 6-pg., 2022-10-17. (19/15333-1, 20/04796-8, 18/22214-6, 21/05665-7, 20/03681-2, 19/12053-8, 20/15098-0)

SEDENHO, GRAZIELA C.; HASSAN, AYAZ; DE SOUZA, JOAO C. P.; CRESPILHO, FRANK N.. In situ and operando electrochemistry of redox enzymes. CURRENT OPINION IN ELECTROCHEMISTRY, v. 34, p. 10-pg., 2022-05-08. (19/12053-8, 19/15333-1, 18/22214-6, 20/04796-8)

CAGNANI, GIOVANA ROSSO; OLIVEIRA, THIAGO DA COSTA; MATTIOLI, ISABELA A.; SEDENHO, GRAZIELA C.; CASTRO, KARLA P. R.; CRESPILHO, FRANK N.. From research to market: correlation between publications, patent filings, and investments in development and production of technological innovations in biosensors. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. N/A, p. 9-pg., 2022-12-07. (19/12053-8, 19/15333-1, 20/04796-8, 18/22214-6, 22/09164-5)

HASSAN, AYAZ; SEDENHO, GRAZIELA C.; VITALE, PHELIPE A. M.; OLIVIERA, MONA N.; CRESPILHO, FRANK N.. On the Weak Binding and Spectroscopic Signature of SARS-CoV-2 nsp14 Interaction with RNA. CHEMBIOCHEM, v. 22, n. 24, OCT 2021. (20/04796-8, 18/22214-6, 19/12053-8, 19/15333-1)

FARIA, LUANA C. I.; SEDENHO, GRAZIELA C.; BERTAGLIA, THIAGO; MACEDO, LUCYANO J. A.; CRESPILHO, FRANK N.. A comparative study of chemically oxidized carbon cloth and thermally treated carbon paper electrodes applied on aqueous organic redox flow batteries. Electrochimica Acta, v. 485, p. 8-pg., 2024-03-16. (22/09164-5, 20/12404-2, 19/21089-6, 20/04796-8, 17/20493-2, 20/03681-2, 18/22214-6)

CLARINDO, JOSE EDUARDO DOS SANTOS; COLOMBO, RAFAEL NERI PRYSTAJ; SEDENHO, GRAZIELA CRISTINA; FARIA, LUANA CRISTINA ITALIANO; BERTAGLIA, THIAGO; LIMA, FILIPE CAMARGO DALMATTI ALVES; GOMES, ROBERTO DA SILVA; AZIZ, MICHAEL J.; CRESPILHO, FRANK NELSON. Molecular Mechanism and Electrostatic Effect Enabling Symmetric All-Quinone Aqueous Redox Flow Batteries. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 12, n. 31, p. 10-pg., 2024-07-23. (22/09164-5, 19/21089-6, 21/05665-7, 19/15333-1, 17/15714-0, 20/04796-8, 15/22973-6, 21/14537-2, 20/03681-2, 18/22214-6, 19/12053-8, 23/17506-6)