Biosensor for dengue detection based on magnetic molecular imprinted nanopolymer

• Grant number: 22/01098-3
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: June 10, 2022
• End date: October 09, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Maria Del Pilar Taboada Sotomayor
• Grantee: Giovana Valero da Silva
• Supervisor: Karin Chumbimuni Torres
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University of Central Florida (UCF), United States
• Associated to the scholarship: 20/01517-0 - Development of a molecularly imprinted polymer (MIP)selective to vitamin k2 for application in food samples, BP.IC

Abstract

Dengue is a disease caused by a flavivirus, which, in addition to classic dengue, can have serious fatal consequences such as dengue hemorrhagic fever. Thus, the development of techniques for better virus detection becomes a very important research area. However, currently, dengue detection methods have limitations, such as the inability to detect in the early stages and high cost. Therefore, the purpose of this research project is the fabrication of an amperometric biosensor based on a Molecularly Imprinted Magnetic Polymer (Mag-MIP), using a specific biotinylated DNA probe for the dengue detection. Biosensors are an excellent option for the detection of dengue, as they have high sensitivity, specificity, and with the use of biomimetic materials (MIP) they will reduce costs to obtain great benefits. Thus, the use of biotinylated probes with DNA from a specific region of dengue, associated with the high molecular recognition capacity of Mag-MIP for biotin, proves to be an advantageous, faster, and low-cost way to detect the dengue virus. (AU)