Evaluation of carbon source on the growth profile of probiotic microorganisms and metabolites produced

• Grant number: 22/03960-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2022
• End date: August 31, 2023
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Michel Brienzo
• Grantee: Mariana Damasceno Pazianoto
• Host Institution: Instituto de Pesquisa em Bioenergia (IPBEN). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated research grant: 17/22401-8 - Fruit-refinery: obtention processes, characterization methods and generation of products derived from residues of the fruitculture, AP.TEM

Abstract

Xylan is the most abundant polysaccharide among the hemicelluloses, with a higher incidence in agricultural and agroindustrial residues, and presents great economic potential. An important application of this macromolecule is the production of xylo-oligosaccharides (XOS): xylose oligomers recognized for their prebiotic effect, in addition to several biological properties beneficial to health. The prebiotic effect of XOS is due to their potential to stimulate the growth and multiplication of probiotic microorganisms at the expense of pathogens due to the production of metabolites such as short-chain fatty acids (SFA). In this study, we intend to evaluate the prebiotic potential of xylo-oligosaccharides with the growth of probiotic microorganisms and also evaluate the profile of metabolites produced by liquid chromatography. XOS will be produced by enzymatic hydrolysis of solubilized xylan from banana pseudostem. The endoxylanase produced by Aspergillus Versicolor will be purified and applied. The XOS will be evaluated at different concentrations to determine the growth profile of the microorganisms Lactiplantibacillus plantarum and Bifidobacterium longum. Similarly, the metabolite products acetate, propionate, and butyrate will be evaluated as a function of the growth stimulation of the microorganisms. The growth of probiotic microorganisms is recognized for its contribution to host health. However, many of the health benefits come from metabolites produced by microorganisms. This study will elucidate the growth profile of probiotic microorganisms by relating it to the metabolites produced. This proposal is inserted in the context of the Fruit-Refining Thematic Project (2017/22401-8), which aims at the utilization of polysaccharides from fruit residues.(AU)