Interaction of Candida streptococcus multispecies biofilm in dental demineralization and oral candidosis

• Grant number: 22/12828-2
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: December 30, 2022
• End date: December 29, 2023
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Ana Cláudia Pavarina
• Grantee: Túlio Morandin Ferrisse
• Supervisor: Hyun Koo
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University of Pennsylvania, United States
• Associated to the scholarship: 21/01191-0 - Tolerance induction and Candida albicans's biofilm characterization after antimicrobial photodymamic therapy application, BP.PD

Abstract

The polymicrobial nature of biofilms associated with oral diseases has increasingly been recognized. These biofilms, through cell-to-cell interactions, cell-extracellular polymeric substances, spatial organization, and biochemical and biophysical components, can modify the microbial virulence capacity. It has already been shown that the Candida albicans-Streptococcus mutans interaction can increases the severity of dental caries lesions. Besides, the interaction between C. albicans-S. gordonni increases tissue invasiveness in oral candidiasis. However, the simultaneous interaction in the dental enamel demineralization process and in oral candidiasis among multispecies biofilms containing C. albicans, S. mutans and S. gordonni has not yet been evaluated. Microbial strains of C. albicans (SC5314), S. mutans (DL1) and S. gordonii will be selected for multispecies biofilm formation in saliva coated hydroxyapatite discs, dental enamel and in an organ-on-chip model of oral keratinocytes. Time-lapse confocal fluorescence microscopy will be used to evaluate the stages of biofilm formation. Multiphoton laser scanning microscopy will be used to evaluate the three-dimensional architecture of the biofilm as well as to verify microbial components and evaluation of proteins related to epithelial integrity (E-cadherin, N-cadherin, Integrin). Non-destructive confocal contrast method, confocal Raman microscopy and Raman spectrometry will be used to evaluate the demineralization capacity of the multispecies biofilm. The analysis and quantification of water and alkali soluble polysaccharides will be carried out. Analysis of pH during multispecies biofilm formation and evaluation of gene expression by qRT-PCR of genes related to virulence of C. albicans (ALS1, EAP1, PHR1, TUP, ECE1, CRZ1) will be performed. Parametric and non-parametric tests of variance will be used for statistical analysis (±=0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; DE ANNUNZIO, SARAH RAQUEL; FRANCA, MARIA GLEIZIANE ARAUJO; SILVA, MARIA GORETTI DE VASCONCELOS; CAVALHEIRO, ALBERTO JOSE; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest. PHARMACEUTICS, v. 15, n. 1, p. 16-pg., 2023-01-01. (13/07600-3, 22/12828-2, 14/50926-0, 20/07110-0, 19/08375-0, 18/09088-1, 18/18440-0, 18/23015-7, 21/01191-0)

FORNEL, DIOGENES GERMANO; FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; FONTANA, CARLA RAQUEL. Photodynamic Therapy Can Modulate the Nasopharyngeal Carcinoma Microenvironment Infected with the Epstein-Barr Virus: A Systematic Review and Meta-Analysis. BIOMEDICINES, v. 11, n. 5, p. 14-pg., 2023-05-02. (22/12828-2, 21/01191-0, 13/07276-1, 20/07110-0)