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| Author(s): |
Marcelo José Dias Silva
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Araraquara. 2016-06-13. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara |
| Defense date: | 2016-04-26 |
| Advisor: | Wagner Vilegas; Marcelo Aparecido da Silva |
| Abstract | |
Mimosa is family Fabaceae and subfamily Mimosaceae, are sources of alkaloids, phenolic acids, terpenoids, carotenoids and flavonoids mainly. Although most species are found in Caatinga and Cerrado, especially in northeastern Brazil, there are few chemical and biological studies on these plants. Therefore, this work contribute knowledge about this family, investigating the leaves of Mimosa caesalpiniifolia indicated against inflammatory, antimicrobial and infections. The extracts were prepared by percolation with 70% ethanol to yield 25% mass. Then they were fractionated using conventional chromatographic techniques. The structures were identified by spectroscopic analysis (UPLC-MS, ESI-IT-MSn and 1H NMR and 13C). The chemical research led to the identification of twenty-seven substances with three flavonoids first described in the literature. In quantification of chemical markers in 100.0 mg of the hydroalcoholic extract of Mimosa (EHM), there are 0.82 mg of ethyl gallate (1) and 0.44 mg cassiaoccidentalina A (21). In the genotoxicity test, the EHM at doses of 62.5 and 125 mg/kg and the ethyl acetate fraction (Fr-EtOAc) at a dose of 62.5 mg/kg showed no toxic effects. The results showed that the EHM was able to prevent oxidative DNA damage in liver cells induced by hydrogen peroxide in vitro and decreased DNA damage was detected in liver cells following exposure to Fr-EtOAc. In the EHM colitis test in a dose of 125 mg/kg showed a preventive effect and Fr-EtOAc 50 mg / kg therapeutic effect with a reduction in the intensity of the lesions. In the test ear edema induced by the EHM xylene (125 and 250 mg/kg) significantly inhibited 52% and 64%, respectively, and exhibited 82% antidiarrheal activity induced by castor oil. In the antifungal assay FR-EtOAc showed IC50 and IC90 of 31,25 and 125 ug/ml against Candida krusei ATCC 6258, and 15.6 and 500 ug/ml against Candida glabrata ATCC 90030. The ethyl gallate substances (1) showed IC50 30 ug/ml against Candida krusei ATCC 6258 and 7.5 ug/ml against Candida glabrata ATCC 90030 mimocaesalpina B (26) showed selectivity for Candida krusei ATCC 6258 at a concentration of 15 ug/ml. These results contributed to the knowledge of the chemical and biological profile of the extracts of the leaves of Mimosa caesalpiniifolia. (AU) | |
| FAPESP's process: | 12/18760-9 - Standardized plant extracts for the treatment of chronic diseases: Mimosa spp. |
| Grantee: | Marcelo José Dias Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |