Importance of genes fliC and motB OF Salmonella enterica subsp. enterica serovar Enteritidis in intestinal colonization and systemic invasion in birds (Gallus gallus domesticus)

Author(s)

Fernanda de Oliveira Barbosa

• Document type: Master's Dissertation
• Press: Jaboticabal. 2016-04-13.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2016-02-29
• Advisor: Angelo Berchieri Junior

Abstract

Salmonella Enteritidis (SE) causes fowl paratyphoid in poultry often related to outbreaks of food-borne diseases in humans. The contribution of flagella and motility in host pathogen interaction require further investigation. To better understand the individual contribution of these virulence factors in poultry, one day old chickens were challenged orally with wildtype strain of SE, a nonmotile but fully flagellated (SE ΔmotB) and aflagellated mutant (SE ΔfliC). Faecal excretion and colonization of liver, spleen and cecal contents by the SE strains were assessed. Additionally, the assessment of gross and microscopic alterations was also performed. At the early stages of infection both mutants showed lower capacity to colonize the ceca, besides the lower recovering in spleen of SE ΔfliC comparing to the wild type of SE. After 7 dpi there were no differences among the counts of the three strains in ceca, liver and spleen. Histopathological analyses demonstrated that flagellated strains (wild type SE and SE ΔmotB) induced lymphoid reactivity in crop, ceca, ileum and liver. On the other hand, in the early stages of infection, SE ΔfliC strain did not stimulate lymphoid reactivity in lamina propria of ceca and ileum but induced discrete necrotic foci in liver. Thus in the present study the flagellar structure and motility seemed to play a role at the early stages of the intestinal colonization and systemic infection by SE in the chicken. (AU)

• FAPESP's process: 14/02014-1 - Importance of genes fliC and motB of Salmonella enterica subsp. enterica serovar Enteritidis in intestinal colonization and systemic invasion in Gallus gallus domesticus
• Grantee: Fernanda de Oliveira Barbosa
• Support Opportunities: Scholarships in Brazil - Master