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Comparative study between generations of transgenic and non-transgenic soybeans seeds using a proteomic and metabolomic approach

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Author(s):
Bruna Kauely de Campos
Total Authors: 1
Document type: Doctoral Thesis
Press: Campinas, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Química
Defense date:
Examining board members:
Marco Aurelio Zezzi Arruda; Ana Rita de Araujo Nogueira; Aline Klassen; Anne Hélène Fostier; Alessandra Sussulini
Advisor: Marco Aurelio Zezzi Arruda
Abstract

This work was based on the comparative study between transgenic (T) and non-transgenic (NT) soybean seeds with respect to their proteins and metabolites in different generations of the plant, since it is believed that the inserted gene can change its profile at the molecular level, once their genome was altered. For this evaluation, it was necessary to plant the soybean seeds in a greenhouse under the same conditions until obtaining the third generation. In addition, several extractions of the proteins of the soybean seeds were carried out for subsequent separation by electrophoresis. The obtained electrophoresis gels show a very similar profile, showing that the separation was adequate, and that there are different proteins and also differentials between them. The relative quantification of the proteins was performed using the 2-D DIGE technique, revealing a total of 89 differential proteins with a selection criterion starting from p<0.1 and a regulation factor greater than 2.0 between the groups studied. A lower number of differential proteins was observed in the T seed groups. After identification by mass spectrometry, 29.2% of the proteins corresponded to the storage class. Regarding the metabolites, the results indicated that the solvent composed of methanol/water (30/70% v/v) was considered the most adequate. Thus, the two seed generations were analyzed by LC-MS with Q-TOF analyser. The obtained data were evaluated by a statistical analysis to better understand the results. The results showed a difference between the T and NT profiles and also between their generations. These results indicate that soybean seeds T and NT are undergoing a change in their proteome and metabolome. The T soybean was more robust, but it is not possible to say if it is due to transgenic. The fact that the smaller differences were observed between the transgenic groups, with a lower number of differential proteins and lower statistical variations with respect to the metabolites, lead us to conclude that transgenic soya is better adapted to the changes that are occurring in its metabolism, whether they are caused by biological, chemical or physical factors (AU)

FAPESP's process: 13/17514-7 - Comparison of Possible Alterations in protein Soybean Seeds and Plants Transgenic and Non-Transgenic Coming from Distinct Generations
Grantee:Bruna Kauely de Campos
Support Opportunities: Scholarships in Brazil - Doctorate