Addition of pomegranate peel extract in a mouthwash with polyphosphate-fluoride reduces the tooth enamel demineralization

Author(s)

Ana Paula Miranda Vieira

• Document type: Doctoral Thesis
• Press: Araçatuba. 2022-12-06.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2022-10-31
• Advisor: Debora de Barros Barbosa

Abstract

Objectives: This study evaluated the anti-demineralizing effect of a mouthwash comprising pomegranate peel extract (PPE 3%), sodium trimetaphosphate (TMP 0.3%), and fluoride (F 225 ppm) in an in situ study and assessed its irritation potential in an ex vivo study. Methods: This double-blind crossover study was conducted in four phases with 7 days each. Twelve volunteers used palatal appliances containing bovine enamel blocks that were subjected to cariogenic challenges with sucrose (30%) six times daily. The ETF formulation (PPE+TMP+F), TF formulation (TMP+F), deionized water, and commercial mouthwash (CM) were dropped onto the enamel blocks twice daily. The percentage of surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), calcium (Ca), phosphorus (P), and F in enamel and biofilms were determined. In addition, alkali-soluble extracellular polysaccharide (EPS) concentrations were analyzed in the biofilms collected from the blocks. The irritation potential of the mouthwashes was evaluated using the hen’s egg chorioallantoic membrane test through the vascular effect produced during 300 s of exposure. Results: The ETF formulation presented the best results for %SH and ΔKHN among all groups (p < 0.001), showed the highest concentrations of Ca and P in the enamel and biofilm, and the lowest amount of EPS in the biofilm (p < 0.001). The ETF produced light reddening, whereas CM led to hyperemia and hemorrhage in the eggs. Significance: The addition of PPE to alcohol-free mouthwash formulations containing TMP and F increased its anti-demineralizing property, and this formulation presented a lower irritation potential than the CM tested. (AU)

• FAPESP's process: 18/06582-5 - Evaluation of the anticariogenic, irritant and cytotoxic potential of an alcohol-free mouthwash formulation containing pomegranate peel extract (Punica granatum), sodium trimetaphosphate and fluorine
• Grantee: Ana Paula Miranda Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate