Natural Green Coating Inhibits Adhesion of Clinically Important Bacteria

Author(s):Show less -

Trentin, Danielle S. ^{[1, 2]} ; Silva, Denise B. ^[3] ; Frasson, Amanda P. ^[1] ; Rzhepishevska, Olena ^[4] ; da Silva, Marcia V. ^{[5, 6]} ; Pulcini, Elinor de L. ; James, Garth ^[7] ; Soares, Gabriel V. ^[8] ; Tasca, Tiana ^[1] ; Ramstedt, Madeleine ^[4] ; Giordani, Raquel B. ^[9] ; Lopes, Norberto P. ^[3] ; Macedo, Alexandre J. ^{[1, 2]}

Affiliation

^[1] Univ Fed Rio Grande do Sul, Fac Farm, BR-90610000 Porto Alegre, RS - Brazil

^[2] Univ Fed Rio Grande do Sul, Ctr Biotecnol, BR-91501970 Porto Alegre, RS - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Nucleo Pesquisas Prod Nat & Sintet NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Umea Univ, Dept Chem, SE-90187 Umea - Sweden

^[5] Univ Fed Pernambuco, Dept Bioquim, BR-50670901 Recife, PE - Brazil

^[6] Univ Fed Pernambuco, Ctr Ciencias Biol, BR-50670901 Recife, PE - Brazil

^[7] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 - USA

^[8] Univ Fed Rio Grande do Sul, Inst Fis, BR-91509900 Porto Alegre, RS - Brazil

^[9] Univ Fed Rio Grande do Norte, Ctr Ciencias Saude, Dept Farm, BR-59010180 Natal, RN - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 5, FEB 6 2015.
• Web of Science Citations: 25

Abstract

Despite many advances, biomaterial-associated infections continue to be a major clinical problem. In order to minimize bacterial adhesion, material surface modifications are currently being investigated and natural products possess large potential for the design of innovative surface coatings. We report the bioguided phytochemical investigation of Pityrocarpa moniliformis and the characterization of tannins by mass spectrometry. It was demonstrated that B-type linked proanthocyanidins-coated surfaces, here termed Green coatings, reduced Gram-positive bacterial adhesion and supported mammalian cell spreading. The proposed mechanism of bacterial attachment inhibition is based on electrostatic repulsion, high hydrophilicity and the steric hindrance provided by the coating that blocks bacterium-substratum interactions. This work shows the applicability of a prototype Green-coated surface that aims to promote necessary mammalian tissue compatibility, while reducing bacterial colonization. (AU)

• FAPESP's process: 14/50265-3 - Distribution and metabolism of natural and synthetic xenobiotics: from the comprehension of reactional process to tissue imaging generation
• Grantee: Norberto Peporine Lopes
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 12/18031-7 - Application of MALDI-MS/MS in the vicenin-2 and derivatives analyses: Understanding the ionization processes to production of the images
• Grantee: Denise Brentan da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/54098-6 - Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances
• Grantee: Norberto Peporine Lopes
• Support Opportunities: Multi-user Equipment Program