Association between Cationic Liposomes and Low Molecular Weight Hyaluronic Acid

Author(s)

Gasperini, Antonio A. M. ^[1] ; Puentes-Martinez, Ximena E. ^{[1, 2]} ; Balbino, Tiago Albertini ^[3] ; Rigoletto, Thais de Paula ^[4] ; Cavalcanti Correa, Gabriela de Sa ^[3] ; Cassago, Alexandre ^[5] ; Portugal, Rodrigo Villares ^[5] ; de La Torre, Lucimara Gaziola ^[3] ; Cavalcanti, Leide P. ^[1]

Affiliation

^[1] CNPEM, Brazilian Synchrotron Light Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, BR-12231280 Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Sch Chem Engn, BR-13083970 Campinas, SP - Brazil

^[4] Ctr Univ Fac Associadas Ensino UNIFAE, BR-13870377 Sao Paulo - Brazil

^[5] CNPEM, Brazilian Nanotechnol Natl Lab, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Langmuir; v. 31, n. 11, p. 3308-3317, MAR 24 2015.
• Web of Science Citations: 12

Abstract

This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential. (AU)

• FAPESP's process: 11/19952-6 - STRUCTURAL CHARACTERIZATION OF AMPHIPHILIC SYSTEMS WITH INDUSTRIAL APPLICATION USING SMALL ANGLE X RAY SCATTERING AND COMPLEMENTARY TECHNIQUES
• Grantee: Antonio Augusto Malfatti Gasperini
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 10/02203-8 - Development of cationic liposomes shielded by hyaluronic acid for DNA vaccination against leishmaniasis
• Grantee: Lucimara Gaziola de la Torre
• Support Opportunities: Regular Research Grants