In vitro evaluation of Sun Protection Factor and stability of commercial sunscreens using mass spectrometry

Author(s)

de Oliveira, Diogo Noin ^[1] ; Delafiori, Jeany ^[1] ; Ferreira, Monica Siqueira ^[1] ; Catharino, Rodrigo Ramos ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Pharmaceut Sci, Innovare Biomarkers Lab, BR-13083877 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 988, p. 13-19, APR 15 2015.
• Web of Science Citations: 1

Abstract

Sunlight exposure causes several types of injury to humans, especially on the skin; among the most common harmful effects due to ultraviolet (UV) exposure are erythema, pigmentation and lesions in DNA, which may lead to cancer. These long-term effects are minimized with the use of sunscreens, a class of cosmetic products that contains UV filters as the main component in the formulation; such molecules can absorb, reflect or diffuse UV rays, and can be used alone or as a combination to broaden the protection on different wavelengths. Currently, worldwide regulatory agencies define which ingredients and what quantities must be used in each country, and enforce companies to conduct tests that confirm the Sun Protection Factor (SPF) and the UVA (Ultraviolet A) factor. Standard SPF determination tests are currently conducted in vivo, using human subjects. In an industrial mindset, apart from economic and ethical reasons, the introduction of an in vitro method emerges as an interesting alternative by reducing risks associated to UV exposure on tests, as well as providing assertive analytical results. The present work aims to describe a novel methodology for SPF determination directly from sunscreen formulations using the previously described cosmetomics platform and mass spectrometry as the analytical methods of choice. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/20066-6 - Cosmetomic application in the development of a new metodology for sunscreens analysis
• Grantee: Jeany Delafiori
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
• Grantee: Aníbal Eugênio Vercesi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/00084-2 - Identification of adulterations and simultaneous determination of nutrients and contaminants in food using direct analysis mass spectrometry and imaging
• Grantee: Diogo Noin de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate