Natural rubber latex coated with calcium phosphate for biomedical application

Author(s)

Borges, Felipe Azevedo ^[1] ; de Almeida Filho, Edson ^[2] ; Romeiro Miranda, Matheus Carlos ^{[3, 1]} ; dos Santos, Marcio Luiz ^[2] ; Herculano, Rondinelli Donizetti ^[3] ; Guastaldi, Antonio Carlos ^[2]

Affiliation

^[1] Sao Paulo State Univ, Biochem & Chem Technol Dept IQ, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ, Phys Chem Dept IQ, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ, Bioproc & Biotechnol Dept FCF, Araraquara, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION; v. 26, n. 17, p. 1256-1268, NOV 22 2015.
• Web of Science Citations: 14

Abstract

Natural rubber latex (NRL) is a flexible biomembrane that possesses angiogenic properties and has recently been used for guided bone regeneration, enhancing healing without fibrous tissue, allergies or rejection. Calcium phosphate (Ca/P) ceramics have chemical, biological, and mechanical properties similar to mineral phase of bone, and ability to bond to the host tissue, although it can disperse from where it is applied. Therefore, to create a composite that could enhance the properties of both materials, NRL biomembranes were coated with Ca/P. NRL biomembranes were soaked in 1.5 times concentrated SBF solution for sevendays, avoiding the use of high temperatures. SEM showed that Ca/P has been coated in NRL biomembrane, XRD showed low crystallinity and FTIR showed that is the carbonated type B. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Ca/P, NRL, and NRL+Ca/P) showed no hemolytic effects up to 0.125mg/mL (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. The results show that the biomimetic is an appropriate method to coat NRL with Ca/P without using high temperatures, aiming a new biomembrane to improve guided bone regeneration. (AU)

• FAPESP's process: 14/17526-8 - Development of a novel system employing natural rubber latex as releasing of vegetable extracts incorporated with nanoparticles: physical chemical and biological characterization
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/17411-8 - Evaluation of drugs delivery systems using natural rubber latex membranes as carrier
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants