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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Self-Assembled Arginine-Capped Peptide Bolaamphiphile Nanosheets for Cell Culture and Controlled Wettability Surfaces

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Author(s):
da Silva, Emerson Rodrigo [1, 2] ; Walter, Merlin Nathaniel Mark [3] ; Reza, Mehedi [4] ; Castelletto, Valeria [1] ; Ruokolainen, Janne [4] ; Connon, Che John [3] ; Alves, Wendel Andrade [2] ; Hamley, Ian William [1]
Total Authors: 8
Affiliation:
[1] Univ Reading, Dept Chem, Reading RG6 6AD, Berks - England
[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil
[3] Newcastle Univ, Inst Med Genet, Int Ctr Life, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear - England
[4] Aalto Univ, Sch Sci, Dept Appl Phys, FI-00076 Aalto - Finland
Total Affiliations: 4
Document type: Journal article
Source: Biomacromolecules; v. 16, n. 10, p. 3180-3190, OCT 2015.
Web of Science Citations: 23
Abstract

The spontaneous assembly of a peptide bolaamphiphile in water, namely, RFL4FR (R, arginine; F, phenylalanine; L, leucine) is investigated, along with its novel properties in surface modification and usage as substrates for cell culture. RFL4FR self-assembles into nanosheets through lateral association of the peptide backbone. The 1,4 sequence is located within the core of the nanosheets, whereas the R moieties are exposed to the water at the surface of the nanosheets. Kinetic assays indicate that the self-assembly is driven by a remarkable two-step process, where a nucleation phase is followed by fast growth of nanosheets with an autocatalysis process. The internal structure of the nanosheets is formed from ultrathin bolaamphiphile monolayers with a crystalline orthorhombic symmetry with cross-beta organization. We show that human corneal stromal fibroblast (hCSF) cells can grow on polystyrene films coated with films dried from RFL4FR solutions. For the first time, this type of amphiphilic peptide is used as a substrate to modulate the wettability of solid surfaces for cell culture applications. (AU)

FAPESP's process: 14/03514-8 - Structural investigations on model-systems based on amphiphilic peptides
Grantee:Emerson Rodrigo da Silva
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 13/12997-0 - Hierarchical self-organization of peptide amphiphiles: fundamental mechanisms and potential applications
Grantee:Wendel Andrade Alves
Support Opportunities: Regular Research Grants