Interaction of SNP in the CRP gene and plasma fatty acid profile in inflammatory pattern: A cross-sectional population-based study

Author(s)

Oki, Erica ^[1] ; Norde, Marina M. ^[1] ; Carioca, Antonio A. F. ^[1] ; Ikeda, Renata E. ^[1] ; Souza, Jose M. P. ^[2] ; Castro, Inar A. ^[3] ; Marchioni, Dirce M. L. ^[1] ; Fisberg, Regina M. ^[1] ; Rogero, Marcelo M. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, BR-05508 Sao Paulo - Brazil

• Document type: Journal article
• Source: NUTRITION; v. 32, n. 1, p. 88-94, JAN 2016.
• Web of Science Citations: 10

Abstract

Objective: To assess the interaction of three single nucleotide polymorphisms in the C-reactive protein (CRP) gene and plasma fatty acid (FA) levels in modulating inflammatory profile. Methods: A total of 262 subjects, aged >19 y and <60 y, participated in a cross-sectional, population-based study performed in Brazil. Three single nucleotide polymorphisms (rs1205, rs1417938, and rs2808630) spanning the CRP gene were genotyped. Eleven plasma inflammatory biomarkers and plasma FA profile were determined. Cluster analysis was performed to stratify individuals based on eleven inflammatory biomarkers into two groups: an inflammatory (INF) and a noninflammatory group. Results: The INF cluster had higher age, waist circumference, systolic blood pressure, and diastolic blood pressure; higher levels of triacylglycerol, high-sensitivity CRP, tumor necrosis factor-alpha, interleukin (IL)-8, IL-6, IL-1p, IL-12, IL-10, soluble monocyte chemoattractant protein-1, soluble intercellular adhesion molecule-1, C16:0, polyunsaturated fatty acid, and omega (n)-6 polyunsaturated fatty acid; and greater C20:4n-6, C18:1/18:0, and C20:4/20:3 ratios than the noninflammatory group. Statistically significant gene-plasma C16:1n-7 interaction was detected for rs1417938 (P = 0.047). Those with a dominant homozygous rs2808630 had a lower risk of belonging to the INF group with the upper 50th percentile of C20:4n-6, n-3 highly unsaturated FA, and C20:4/20:3 ratio. Regarding rs1205, A allele carriers had lower risk of being in the INF group when C20:5n-3 and n-3 highly unsaturated FA levels were greater than the median. Conclusions: The INF group exhibited changes in metabolic parameters that predispose this group to chronic disease, where polymorphisms in the CRP gene modulated the risk of being in the INF group depending on individual plasma fatty acid and lipid profile. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 12/20401-7 - Association between SNP related to adiponectin, C-reactive protein, Toll Like Receptor 4, TNF-alfa, IL-1 beta, IL-6 and IL-10 genes and lipids intake and their effects on plasma inflammatory biomarkers levels in population-based study
• Grantee: Marcelo Macedo Rogero
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/01740-8 - Association between single nucleotide polymorphism related to adiponectin, Toll like receptor 4, IL-1² and IL-6 genes and fatty acids intake and their effects on systemic inflammatory pattern in a population based study - ISA capital.
• Grantee: Marina Maintinguer Norde
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/15831-0 - Dietary factors, homocystein, MTHFR gene polymorphisms, and cardiovascular risk in adults and the elderly: a population-based study - ISA - Capital
• Grantee: Regina Mara Fisberg
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/01741-4 - Association between single nucleotide polymorphism in genes of CRP, TNF-alfa and IL-10 and plasma fatty acids and their effect to a systemic inflammatory patter at a population-based study - ISA-Capital
• Grantee: Érica Oki
• Support Opportunities: Scholarships in Brazil - Master