| Grant number: | 09/15831-0 |
| Support Opportunities: | Regular Research Grants |
| Start date: | January 01, 2010 |
| End date: | December 31, 2011 |
| Field of knowledge: | Health Sciences - Nutrition - Nutritional Analysis of Population |
| Principal Investigator: | Regina Mara Fisberg |
| Grantee: | Regina Mara Fisberg |
| Host Institution: | Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
Abstract
In recent years clinical and epidemiological studies have evidenced plasma levels of homocystein are associated to increased risk of atherosclerosis and ischemic cardiovascular events. Among adults, blood homocystein shows specific characteristics and distribution according to age and gender. But, there are scarce Brazilian studies investigating this subject in representative samples of general population. The objective of the present study is to assess the association between cardiovascular risk factors and blood levels of homocystein, MTHFR gene polymorphisms and dietary factors in a probabilistic sample of subjects included in the second phase of ISA study, a population-based household health survey in the city of São Paulo. Demographic, socioeconomic and lifestyle information including diet, physical activity, smoking and alcohol abuse will be collected as well as data on blood pressure, anthropometric measures, and venous blood samples. Food intake information will be collected at two different opportunities using a 24-hour food recall (24HR). Cholesterol and its fractions, homocystein, folate, vitamin B12 and B16 and biomarkers of inflammatory response involved in atherosclerosis etiology will be measured in the blood drawn. DNA will be extracted and amplified by PCR using specific primers for two MTHFR polymorphisms (A1298C and C677T). The product will undergo digestion using Hinf I enzyme (New England BioLabs) for C677T and Mbo II (New England BioLabs) for A128C. All digestion products will be assayed in 3% agarose gel, and ethidium bromide stained. The associations of interest will be analyzed using non-conditional logistic regression or linear regression. (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
| More itemsLess items |
| TITULO |
| Articles published in other media outlets ( ): |
| More itemsLess items |
| VEICULO: TITULO (DATA) |
| VEICULO: TITULO (DATA) |