Fast analysis of curcuminoids from turmeric (Curcuma longa L.) by high-performance liquid chromatography using a fused-core column

Author(s)

Felipe Osorio-Tobon, J. ^[1] ; Carvalho, Pedro I. N. ^[1] ; Fernandez Barbero, Gerardo ^[2] ; Nogueira, Gislaine Chrystina ^[1] ; Rostagno, Mauricio Ariel ^[3] ; de Almeida Meireles, Maria Angela ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Food Engn FEA, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Cadiz, Fac Sci, Dept Analyt Chem, Agrifood Campus Int Excellence CeiA3, POB 40, Cadiz 11510 - Spain

^[3] Univ Estadual Campinas, Sch Appl Sci FCA, R Pedro Zaccaria 1300, BR-13484350 Sao Paulo - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 200, p. 167-174, JUN 1 2016.
• Web of Science Citations: 20

Abstract

The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of a fast method for the analysis of main curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) present in extracts of turmeric (Curcuma longa L.). A step-by-step strategy was used to optimize temperature (40-55 degrees C), flow rate (1.0-2.5 mL min (1)), mobile phase composition and equilibration time (1-5 min). A gradient method was developed using acidified water and acetonitrile combined with high column temperature (55 degrees C) and flow rate (2.5 mL min (1)). Optimized conditions provided a method for the separation of these three curcuminoids in approximately 1.3 min with a total analysis time (sample-to-sample) of 7 min, including the clean-up and the re-equilibration of the column. Evaluation of chromatographic performance revealed excellent intraday and interday reproducibility (>99%), resolution (>2.23), selectivity (>1.12), peak symmetry (1.24-1.42) while presenting low limits of detection (<0.40 mg L (1)) and quantification (<1.34 mg L (1)). The robustness of the method was calculated according to the concentration/dilution of the sample and the injection volume. Several combinations of methanol and ethanol with water as sample solvents were evaluated and the best chromatographic results and extraction rate were obtained using 100% methanol. Finally, the developed method was validated with different extracts of turmeric rhizome and products that use turmeric in their formulation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 12/10685-8 - Combination and coupling of processes for the production and encapsulation of value-added vegetal extracts: technical and economic evaluation
• Grantee: Maria Angela de Almeida Meireles Petenate
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/20758-5 - Volatile oil extraction from turmeric by supercritical carbon dioxide: study of the extraction in beds of different geometries and operational configurations
• Grantee: Pedro Ivo Nunes de Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)