Dextran-Coated Antiferromagnetic MnO Nanoparticles for a T-1-MRI Contrast Agent with High Colloidal Stability

Author(s)

Neves, Herbert R. ^[1] ; Bini, Rafael A. ^[2] ; Barbosa, Jeam H. O. ^[3] ; Salmon, Carlos E. G. ^[3] ; Varanda, Laudemir C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Chem Inst Sao Carlos, Colloidal Mat Grp, BR-13566590 Sao Carlos, SP - Brazil

^[2] Fed Univ Technol UTFPR, Campus Toledo, BR-85902490 Toledo, Parana - Brazil

^[3] Univ Sao Paulo, FFCLRP, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION; v. 33, n. 3, p. 167-176, MAR 2016.
• Web of Science Citations: 4

Abstract

A simple approach to synthesize carboxymethyl dextran-coated MnO nanoparticles (CMDex-MnONPs) with high colloidal stability in physiological saline solutions is described here for potential applications as a magnetic resonance imaging (MRI) T-1 contrast agent. The thermal decomposition methodology is used to produce uniform MnONPs with an average size of around 20 nm, and its hydrophobic surface is modified with CMDex molecules, conferring hydrophilic properties. After CMDex coating, the nanoparticle presents high colloidal stability in concentrations ranging from 10 to 50 mu g mL(-1), average hydrodynamic size (Z-average) of 130 nm, polydispersity degree of approximate to 12%, and negative surface charge in both simulated body fluid solutions and pure water with zeta-potential of -20 and -40 mV, respectively. The CMDex-MnONPs with 20 nm show antiferromagnetic behavior at room temperature, and the magnetic properties are found to be strongly dependent of the nanoparticle size, increasing the contribution of the ferromagnetic Mn3O4 phase with decreasing size for nanoparticles about 3 nm. Cytotoxicity evaluation in cancerous and noncancerous cells in the range of 5.0-50.0 mu g mL(-1) shows low toxicity for cancerous cells and lack of the same for healthy cells lines. Related to the magnetic properties, CMDex-MnONP presents significant r(1) relaxivity and low r(2)/r(1) relaxivity ratio. The results suggest that these nanoparticles display characteristics for potential applications as an MRI T-1 contrast agent. (AU)

• FAPESP's process: 13/01284-2 - Theranostic nanoparticles based in superparamagnetic iron oxide for skin cancer therapy by magnetic hyperthermia
• Grantee: Laudemir Carlos Varanda
• Support Opportunities: Regular Research Grants
• FAPESP's process: 07/07919-9 - Colloidal magnetic nanocrystals: synthesis of self-assembled and macromolecules-functionalised nanospheres, nanowires, and nanorods for advanced magnetic recording, biotechnological, and biomedical applications
• Grantee: Laudemir Carlos Varanda
• Support Opportunities: Research Grants - Young Investigators Grants