Endophytic Actinobacteria from the Brazilian Medicinal Plant Lychnophora ericoides Mart. and the Biological Potential of Their Secondary Metabolites

Author(s):Show less -

Conti, Raphael ^{[1, 2]} ; Chagas, Fernanda Oliveira ^{[1, 2]} ; Caraballo-Rodriguez, Andres Mauricio ^{[1, 2]} ; da Paixao Melo, Weilan Gomes ^{[1, 2]} ; do Nascimento, Andrea Mendes ^[3] ; Cavalcanti, Bruno Coelho ^[4] ; de Moraes, Manoel Odorico ^[4] ; Pessoa, Claudia ^{[4, 5]} ; Costa-Lotufo, Leticia Veras ^[6] ; Krogh, Renata ^{[7, 2]} ; Andricopulo, Adriano Defini ^{[7, 2]} ; Lopes, Norberto Peporine ^[1] ; Pupo, Monica Tallarico ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] FAPESP, CEPID CIBFar Ctr Pesquisa & Inovacao Biodiversida, Sao Paulo, SP - Brazil

^[3] Univ Fed Ouro Preto, Inst Ciencias Exatas & Biol, Ouro Preto, MG - Brazil

^[4] Univ Fed Ceara, Fac Med, Fortaleza, Ceara - Brazil

^[5] Fundacao Oswaldo Cruz Fiocruz, Fortaleza, Ceara - Brazil

^[6] Univ Sao Paulo, Inst Ciencias Biomed, Sao Paulo, SP - Brazil

^[7] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: CHEMISTRY & BIODIVERSITY; v. 13, n. 6, p. 727-736, JUN 2016.
• Web of Science Citations: 12

Abstract

Endophytic actinobacteria from the Brazilian medicinal plant Lychnophora ericoides were isolated for the first time, and the biological potential of their secondary metabolites was evaluated. A phylogenic analysis of isolated actinobacteria was accomplished with 16S rRNA gene sequencing, and the predominance of the genus Streptomyces was observed. All strains were cultured on solid rice medium, and ethanol extracts were evaluated with antimicrobial and cytotoxic assays against cancer cell lines. As a result, 92% of the extracts showed a high or moderate activity against at least one pathogenic microbial strain or cancer cell line. Based on the biological and chemical analyses of crude extracts, three endophytic strains were selected for further investigation of their chemical profiles. Sixteen compounds were isolated, and 3-hydroxy-4-methoxybenzamide (9) and 2,3-dihydro-2,2-dimethyl-4(1H)-quinazolinone (15) are reported as natural products for the first time in this study. The biological activity of the pure compounds was also assessed. Compound 15 displayed potent cytotoxic activity against all four tested cancer cell lines. Nocardamine (2) was only moderately active against two cancer cell lines but showed strong activity against Trypanosoma cruzi. Our results show that endophytic actinobacteria from L.ericoides are a promising source of bioactive compounds. (AU)

• FAPESP's process: 08/00812-7 - Evaluation of the biotechnological potential of endophytic actinobacteria isolated from Lychnophora ericoides ("falsa-arnica")
• Grantee: Raphael Conti
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 08/09540-0 - New strategies in the search of natural antibiotics: symbiotic microorganisms as sources of compounds and in vivo assay based on Caenorhabditis elegans infection model
• Grantee: Mônica Tallarico Pupo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 09/17695-6 - New strategies to access microbial natural products: mixed cultures of endophytes, chemical and epigenetic remodeling of biosynthetic pathways
• Grantee: Fernanda Oliveira das Chagas
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/21803-1 - Elicitation of natural products biosynthesis by endophytic microorganisms’ interaction
• Grantee: Andrés Mauricio Caraballo Rodríguez
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/01001-6 - Discovering bacterial symbionts diversity associated with ants in different Brazilian biomes
• Grantee: Weilan Gomes da Paixão Melo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral