| Full text | |
| Author(s): Show less - |
Andrade-Sousa, Adilson Santos
;
Pereira, Paulo Rogerio
;
MacKenzie, BreAnne
;
Oliveira-Junior, Manoel Carneiro
;
Assumpcao-Neto, Erasmo
;
Rodrigues Brandao-Rangel, Maysa Alves
;
Damaceno-Rodrigues, Nilsa Regina
;
Caldini, Elia Garcia
;
Pereira Velosa, Ana Paula
;
Teodoro, Walcy Rosolia
;
Ligeiro de Oliveira, Ana Paula
;
Dolhnikoff, Marisa
;
Eickelberg, Oliver
;
Vieira, Rodolfo Paula
Total Authors: 14
|
| Document type: | Journal article |
| Source: | PLoS One; v. 11, n. 9 SEP 27 2016. |
| Web of Science Citations: | 5 |
| Abstract | |
Introduction The aim of this study was to investigate the effect of aerobic exercise (AE) in reducing bleomycin- induced fibrosis in mice of a Th2-dominant immune background (BALB/c). Methods BALB/c mice were distributed into: sedentary, control (CON), Exercise-only (EX), sedentary, bleomycin-treated (BLEO) and bleomycin-treated+exercised (BLEO+EX); (n = 8/group). Following treadmill adaptation, 15 days following a single, oro-tracheal administration of bleomycin (1.5U/kg), AE was performed 5 days/week, 60min/day for 4 weeks at moderate intensity (60% of maximum velocity reached during a physical test) and assessed for pulmonary inflammation and remodeling, and cytokine levels in bronchoalveolar lavage (BAL). Results At 45 days post injury, compared to BLEO, BLEO+EX demonstrated reduced collagen deposition in the airways (p<0.001) and also in the lung parenchyma (p<0.001). In BAL, a decreased number of total leukocytes (p<0.01), eosinophils (p<0.001), lymphocytes (p<0.01), macrophages (p<0.01), and neutrophils (p<0.01), as well as reduced pro-inflammatory cytokines (CXCL-1; p<0.01), (IL-1 beta; p<0.001), (IL-5; p<0.01), (IL-6; p<0.001), (IL-13; p<0.01) and pro-fibrotic growth factor IGF-1 (p<0.001) were observed. Anti-inflammatory cytokine IL-10 was increased (p<0.001). Conclusion AE attenuated bleomycin-induced collagen deposition, inflammation and cytokines accumulation in the lungs of mice with a predominately Th2-background suggesting that therapeutic AE (15-44 days post injury) attenuates the pro-inflammatory, Th2 immune response and fibrosis in the bleomycin model. (AU) | |
| FAPESP's process: | 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals |
| Grantee: | Rodolfo de Paula Vieira |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 14/14604-8 - Role of purinergic signaling in the antiinflammatory effects of aerobic training in a model of asthma induced by house dust mite |
| Grantee: | Manoel Carneiro de Oliveira Junior |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/23196-0 - The role of purinergic signalling and of SOCS-JAK-STAT signalling in the anti-inflammatory effects of aerobic training in asthmatic individuals. |
| Grantee: | Breanne Mackenzie |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/51464-4 - Effects of aerobic exercise on pulmonary fibrosis induced by bleomycin. (fapesp/baylat) |
| Grantee: | Rodolfo de Paula Vieira |
| Support Opportunities: | Regular Research Grants |