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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Effects of Low-Power Laser Irradiation on Inflammation and Apoptosis in Submandibular Glands of Diabetes-Induced Rats

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Author(s):
Fukuoka, Cintia Yuki ; Simoes, Alyne ; Uchiyama, Toshikazu ; Arana-Chavez, Victor Elias ; Abiko, Yoshimitsu ; Kuboyama, Noboru ; Bhawal, Ujjal K.
Total Authors: 7
Document type: Journal article
Source: PLoS One; v. 12, n. 1 JAN 18 2017.
Web of Science Citations: 6
Abstract

Diabetes can lead to dysfunction of the secretory capacity in salivary glands. Activation of the receptor for advanced glycation end products (RAGE) and its ligands has been suggested to participate in chronic disorders such as diabetes and its complications. In this study, the expression of RAGE, high mobility group box 1 (HMGB1) and advanced glycation end products (AGE), as well as the effects of low-power laser irradiation (LPLI) in salivary glands of diabetic rats were evaluated, and the mechanisms involved were characterized. The expression of RAGE and HMGB1 at the protein and mRNA levels was observed in sub mandibular glands (SMGs) of streptozotocin-induced diabetic rats. A diode laser was applied at 660 nm, 70 mW, 20 J/cm(2), 0.56 J/point, with a spot area of 0.028 cm(2) and its in vivo effects and the pathways involved were evaluated. lmmunohistochemistry and western blotting analysis were performed for inflammatory and apoptosis markers. Diabetes up-regulates HMGB1/AGE/RAGE axis gene expression in SMGs that is associated with activation of the nuclear factor kappa B (NF-kappa B) pathway. Interestingly, LPLI suppresses NF-kappa B activation induced by inflammation. LPLI also reduces diabetes-induced apoptosis. That effect was accompanied by decreased levels of Bax, and cleaved caspase 3, which were up-regulated in diabetes. Taken together, our data suggest that LPLI reduces diabetes-induced inflammation by reducing the induction of HMGB1, ultimately leading to inhibition of apoptosis in submandibular glands of diabetic rats. (AU)

FAPESP's process: 14/21214-1 - Biomodulation effect of low level laser therapy in diabetes: action mechanism and dosimetry
Grantee:Alyne Simões Gonçalves
Support Opportunities: Regular Research Grants