One-pot preparation of PEDOT:PSS-reduced graphene decorated with Au nanoparticles for enzymatic electrochemical sensing of H2O2

Author(s)

Mercante, Luiza A. ; Facure, Murilo H. M. ; Sanfelice, Rafaela C. ; Migliorini, Fernanda L. ; Mattoso, Luiz H. C. ; Correa, Daniel S.

• Document type: Journal article
• Source: Applied Surface Science; v. 407, p. 162-170, JUN 15 2017.
• Web of Science Citations: 33

Abstract

The development of novel graphene-based nanocomposites is a hotspot in materials science due to their unique optical, electronic, thermal, mechanical and catalytic properties for varied applications. The present work reports on the development of a graphene-based ternary nanocomposite of poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate), reduced graphene oxide and gold nanopartides (PEDOT:PSS-rGO-AuNPs) for the detection of hydrogen peroxide (H2O2). The hybrid nanocomposite showed superior electrochemical properties and higher stability compared to each individual component as electrode materials, showing a synergistic effect between PEDOT, rGO and AuNPs. The nanocomposite was obtained via a facile one-step approach and was assembly with horseradish peroxidase (HRP). The PEDOT:PSS-rGO-AuNPs-HRP modified electrode has been used for the amperometric detection of H2O2 and exhibited a high sensitivity of up to 677 mu A mM(-1) cm(-2). with a wide linear range from S to 400 mu M and a low detection limit of 0.08 mu M (S/N=3). This developed enzymatic biosensor showed to be highly stable and unresponsive to potentially interfering substances, and it could be used for sensing H2O2 in real samples, including tap water and bovine milk samples. These enhanced sensing performance could be ascribed to the intimate contact of AuNPs onto the rough surface of the PEDOT:PSS-rGO nanocomposite, which has a high electrical conductivity and large surface area, providing it as an excellent substrate for the growth and support of nanoparticles. The method developed in this work opens up a general route to prepare a wide range of graphene-based hybrid nanocomposite films with multiple functions including sensing and biosensing. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/23880-3 - Development of polymeric nanostructures as a sensor platform for diagnosis of bacteria causing mastitis
• Grantee: Luiza Amim Mercante
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/13140-0 - Production and characterization of hybrid materials based on graphene/conductive polymer and applications in electronic tongue type chemical sensors
• Grantee: Murilo Henrique Moreira Facure
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/16789-5 - Hybrid nanostructured materials based on conjugated polymers and metallic nanoparticles for sensor applications
• Grantee: Daniel Souza Corrêa
• Support Opportunities: Regular Research Grants