Sources, targets and biological responses of triplet species and singlet oxygen: p...
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| Author(s): Show less - |
Kaskova, Zinaida M.
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Dorr, Felipe A.
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Petushkov, Valentin N.
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Purtov, Konstantin V.
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Tsarkova, Aleksandra S.
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Rodionova, Natalja S.
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Mineev, Konstantin S.
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Guglya, Elena B.
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Kotlobay, Alexey
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Baleeva, Nadezhda S.
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Baranov, Mikhail S.
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Arseniev, Alexander S.
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Gitelson, Josef I.
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Lukyanov, Sergey
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Suzuki, Yoshiki
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Kanie, Shusei
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Pinto, Ernani
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Di Mascio, Paolo
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Waldenmaier, Hans E.
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Pereira, Tatiana A.
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Carvalho, Rodrigo P.
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Oliveira, Anderson G.
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Oba, Yuichi
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Bastos, Erick L.
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Stevani, Cassius V.
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Yampolsky, Ilia V.
Total Authors: 26
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| Document type: | Journal article |
| Source: | SCIENCE ADVANCES; v. 3, n. 4 APR 2017. |
| Web of Science Citations: | 19 |
| Abstract | |
Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates. (AU) | |
| FAPESP's process: | 10/11578-5 - Purification and cloning of a reductase and luciferase from bioluminescent fungi |
| Grantee: | Anderson Garbuglio de Oliveira |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 13/16885-1 - Fungal bioluminescence: species survey, mechanistic study & toxicological assays |
| Grantee: | Cassius Vinicius Stevani |
| Support type: | Regular Research Grants |
| FAPESP's process: | 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology |
| Grantee: | Paolo Di Mascio |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/07914-8 - FoRC - Food Research Center |
| Grantee: | Bernadette Dora Gombossy de Melo Franco |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 14/14866-2 - Intermolecular interactions involving betalains |
| Grantee: | Erick Leite Bastos |
| Support type: | Regular Research Grants |