Mechanism and color modulation of fungal bioluminescence

Author(s):Show less -

Kaskova, Zinaida M. ; Dorr, Felipe A. ; Petushkov, Valentin N. ; Purtov, Konstantin V. ; Tsarkova, Aleksandra S. ; Rodionova, Natalja S. ; Mineev, Konstantin S. ; Guglya, Elena B. ; Kotlobay, Alexey ; Baleeva, Nadezhda S. ; Baranov, Mikhail S. ; Arseniev, Alexander S. ; Gitelson, Josef I. ; Lukyanov, Sergey ; Suzuki, Yoshiki ; Kanie, Shusei ; Pinto, Ernani ; Di Mascio, Paolo ; Waldenmaier, Hans E. ; Pereira, Tatiana A. ; Carvalho, Rodrigo P. ; Oliveira, Anderson G. ; Oba, Yuichi ; Bastos, Erick L. ; Stevani, Cassius V. ; Yampolsky, Ilia V.

• Document type: Journal article
• Source: SCIENCE ADVANCES; v. 3, n. 4 APR 2017.
• Web of Science Citations: 20

Abstract

Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates. (AU)

• FAPESP's process: 14/14866-2 - Intermolecular interactions involving betalains
• Grantee: Erick Leite Bastos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology
• Grantee: Paolo Di Mascio
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
• Grantee: Ohara Augusto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 10/11578-5 - Purification and cloning of a reductase and luciferase from bioluminescent fungi.
• Grantee: Anderson Garbuglio de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/16885-1 - Fungal bioluminescence: species survey, mechanistic study & toxicological assays
• Grantee: Cassius Vinicius Stevani
• Support Opportunities: Regular Research Grants