| Full text | |
| Author(s): |
Santana-Melo, Gabriela F.
;
Rodrigues, Bruno V. M.
;
da Silva, Edmundo
;
Ricci, Ritchelli
;
Marciano, Fernanda R.
;
Webster, Thomas J.
;
Vasconcellos, Luana M. R.
;
Lobo, Anderson O.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 155, p. 544-552, JUL 1 2017. |
| Web of Science Citations: | 15 |
| Abstract | |
Combining polyester scaffolds with synthetic nanohydroxyapatite (nHAp), which is bioactive and osteoconductive, is a plausible strategy to improve bone regeneration. Here, we propose the combination of PBAT {[}poly(butylene-adipate-co-terephthalate)] and synthetic nHAp (at 3 and 5 wt%). PBAT is a relatively a new polymer with low crystallinity and attractive biodegradability and mechanical properties for orthopedic applications, however, with a still underexplored potential for in vivo applications. Then, we performed a careful biological in vitro and in vivo set of experiments to evaluate the influence of PBAT containing two different nHAp loads. For in vitro assays, osteoblast-like MG63 cells were used and the bioactivity and gene expression related to osteogenesis were evaluated by qRT-PCR. For in vivo experiments, twenty-four male rats were used and a tibial defect model was applied to insert the scaffolds. Micro-computed tomography (Micro-CT) and histological analysis were used to assess e bone neoformation after 6 weeks of implantation. Three point flexural tests measured the mechanical properties of the neoformed bone. All scaffolds showed promising in vitro properties, since they were not cytotoxic against MG-63 cells and promoted high cell proliferation and formation of mineralized nodules. From a mechanistic point-of-view, nHAp loading increased hydrophilicity, which in turn allowed for a better adsorption of proteins and consequent changes in the phenotypic expression of osteoblasts. nHAp induced better cellular responses on/in the scaffolds, which was mainly attributed to its osteoconductive and osteoinductive properties. Micro-CT images showed that nHAp at 3% and 5 wt% led to more effective bone formation, presenting the highest bone volume after 6 weeks of implantation. Considering the three point flexural tests, 5 wt% of nHAp positively influenced the flexural mode of the neoformed bone, but the stiffness was similar between the 3% and 5 wt% groups. In summary, this investigation demonstrated great potential for the application of these novel scaffolds towards bone regeneration and, thus, should be further studied. (C) 2017 Elsevier B.V. All rights reserved, (AU) | |
| FAPESP's process: | 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration |
| Grantee: | Anderson de Oliveira Lobo |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications |
| Grantee: | Fernanda Roberta Marciano |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/09697-0 - Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering |
| Grantee: | Anderson de Oliveira Lobo |
| Support Opportunities: | Scholarships abroad - Research |
| FAPESP's process: | 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers |
| Grantee: | Bruno Vinícius Manzolli Rodrigues |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/00575-1 - Functional biomembranes based on peptides and bottlebrush polymers to tissue engineering |
| Grantee: | Fernanda Roberta Marciano |
| Support Opportunities: | Scholarships abroad - Research |