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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

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Author(s):
Santana-Melo, Gabriela F. ; Rodrigues, Bruno V. M. ; da Silva, Edmundo ; Ricci, Ritchelli ; Marciano, Fernanda R. ; Webster, Thomas J. ; Vasconcellos, Luana M. R. ; Lobo, Anderson O.
Total Authors: 8
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 155, p. 544-552, JUL 1 2017.
Web of Science Citations: 15
Abstract

Combining polyester scaffolds with synthetic nanohydroxyapatite (nHAp), which is bioactive and osteoconductive, is a plausible strategy to improve bone regeneration. Here, we propose the combination of PBAT {[}poly(butylene-adipate-co-terephthalate)] and synthetic nHAp (at 3 and 5 wt%). PBAT is a relatively a new polymer with low crystallinity and attractive biodegradability and mechanical properties for orthopedic applications, however, with a still underexplored potential for in vivo applications. Then, we performed a careful biological in vitro and in vivo set of experiments to evaluate the influence of PBAT containing two different nHAp loads. For in vitro assays, osteoblast-like MG63 cells were used and the bioactivity and gene expression related to osteogenesis were evaluated by qRT-PCR. For in vivo experiments, twenty-four male rats were used and a tibial defect model was applied to insert the scaffolds. Micro-computed tomography (Micro-CT) and histological analysis were used to assess e bone neoformation after 6 weeks of implantation. Three point flexural tests measured the mechanical properties of the neoformed bone. All scaffolds showed promising in vitro properties, since they were not cytotoxic against MG-63 cells and promoted high cell proliferation and formation of mineralized nodules. From a mechanistic point-of-view, nHAp loading increased hydrophilicity, which in turn allowed for a better adsorption of proteins and consequent changes in the phenotypic expression of osteoblasts. nHAp induced better cellular responses on/in the scaffolds, which was mainly attributed to its osteoconductive and osteoinductive properties. Micro-CT images showed that nHAp at 3% and 5 wt% led to more effective bone formation, presenting the highest bone volume after 6 weeks of implantation. Considering the three point flexural tests, 5 wt% of nHAp positively influenced the flexural mode of the neoformed bone, but the stiffness was similar between the 3% and 5 wt% groups. In summary, this investigation demonstrated great potential for the application of these novel scaffolds towards bone regeneration and, thus, should be further studied. (C) 2017 Elsevier B.V. All rights reserved, (AU)

FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/09697-0 - Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering
Grantee:Anderson de Oliveira Lobo
Support type: Scholarships abroad - Research
FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers
Grantee:Bruno Vinícius Manzolli Rodrigues
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/00575-1 - Functional biomembranes based on peptides and bottlebrush polymers to tissue engineering
Grantee:Fernanda Roberta Marciano
Support type: Scholarships abroad - Research