Polymorphisms of the TNF-alpha gene interact with plasma fatty acids on inflammatory biomarker profile: a population-based, cross-sectional study in Sao Paulo, Brazil

Author(s)

Oki, Erica ; Norde, Marina N. ; Carioca, Antonio A. F. ; Souza, Jose M. P. ; Castro, Inar A. ; Marchioni, Dirce M. L. ; Fisberg, Regina M. ; Rogero, Marcelo M.

• Document type: Journal article
• Source: BRITISH JOURNAL OF NUTRITION; v. 117, n. 12, p. 1663-1673, JUN 2017.
• Web of Science Citations: 4

Abstract

The aim of the present study was to investigate the relationship of four TNF-alpha SNP with inflammatory biomarkers and plasma fatty acids (FA), and the interaction among them in a population-based, cross-sectional study in Sao Paulo, Brazil. A total of 281 subjects, aged > 19 and < 60 years, participated in a cross-sectional, population-based study performed in Brazil. The following SNP spanning the TNF-alpha gene were genotyped: -238G/A (rs361525), -308G/A (rs1800629), -857C/T (rs1799724) and -1031T/C (rs1799964). In all, eleven plasma inflammatory biomarkers and plasma FA profile were determined. To analyse the interaction between TNF-alpha SNP and plasma FA, a cluster analysis was performed to stratify individuals based on eleven inflammatory biomarkers into two groups used as outcome: inflammatory (INF) and non-inflammatory clusters. The -238A allele carriers had higher TNF-alpha (P=0.033), IL-6 (P=0.013), IL-1 beta (P=0.037), IL-12 (0.048) and IL-10 (P=0.010) than the GG genotype. The -308A allele carriers also had lower levels of plasma palmitoleic acid (P=0.009), oleic acid (P=0.039), total MUFA (P=0.014), stearoyl-CoA desaturase (SCD) activity index-16 (P=0.007), SCD-18 (P=0.020) and higher levels of PUFA (P=0.046) and DHA (P=0.044). Significant interactions modifying the risk of belonging to the INF cluster were observed with inflammatory cluster as outcome between -857C/T and plasma alpha-linolenic acid (P=0.026), and also between -308G/A and plasma stearic acid (P=0.044) and total SFA (P=0.040). Our study contributes to knowledge on TNF-alpha SNP and their association with inflammatory biomarker levels, plasma FA and the interaction among them, of particular interest for the Brazilian population. (AU)

• FAPESP's process: 14/16347-2 - Metabolic signature on migrants and their relationship to patterns of consumption and metabolic syndrome: an epidemiological approach to elucidate the effects of diet
• Grantee: Antonio Augusto Ferreira Carioca
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/20401-7 - Association between SNP related to adiponectin, C-reactive protein, Toll Like Receptor 4, TNF-alfa, IL-1 beta, IL-6 and IL-10 genes and lipids intake and their effects on plasma inflammatory biomarkers levels in population-based study
• Grantee: Marcelo Macedo Rogero
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/01741-4 - Association between single nucleotide polymorphism in genes of CRP, TNF-alfa and IL-10 and plasma fatty acids and their effect to a systemic inflammatory patter at a population-based study - ISA-Capital
• Grantee: Érica Oki
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/01740-8 - Association between single nucleotide polymorphism related to adiponectin, Toll like receptor 4, IL-1² and IL-6 genes and fatty acids intake and their effects on systemic inflammatory pattern in a population based study - ISA capital.
• Grantee: Marina Maintinguer Norde
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/15831-0 - Dietary factors, homocystein, MTHFR gene polymorphisms, and cardiovascular risk in adults and the elderly: a population-based study - ISA - Capital
• Grantee: Regina Mara Fisberg
• Support Opportunities: Regular Research Grants