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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In-vivo electrochemical monitoring of H2O2 production induced by root-inoculated endophytic bacteria in Agave tequilana leaves

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Lima, Alex S. [1] ; Prieto, Katia R. [2] ; Santos, Carla S. [1] ; Valerio, Hellen Paula [2] ; Garcia-Ochoa, Evelyn Y. [3] ; Huerta-Robles, Aurora [4] ; Beltran-Garcia, Miguel J. [3] ; Di Mascio, Paolo [2] ; Bertotti, Mauro [1]
Total Authors: 9
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Autonoma Guadalajara, Dept Chem ICET, Patria 1201, Zapopan, Jalisco - Mexico
[4] Univ Autonoma Baja California, Inst Engn, Blvd B Juarez & Calle Normal S-N, Mexicali, BC - Mexico
Total Affiliations: 4
Document type: Journal article
Source: BIOSENSORS & BIOELECTRONICS; v. 99, p. 108-114, JAN 15 2018.
Web of Science Citations: 9

A dual-function platinum disc microelectrode sensor was used for in-situ monitoring of H2O2 produced in A. tequilana leaves after inoculation of their endophytic bacteria (Enterobacter cloacae). Voltammetric experiments were carried out from 0.0 to -1.0 V, a potential range where H2O2 is electrochemically reduced. A needle was used to create a small cavity in the upper epidermis of A. tequilana leaves, where the fabricated electrochemical sensor was inserted by using a manual three-dimensional micropositioner. Control experiments were performed with untreated plants and the obtained electrochemical results clearly proved the formation of H2O2 in the leaves of plants 3 h after the E. cloacae inoculation, according to a mechanism involving endogenous signaling pathways. In order to compare the sensitivity of the microelectrode sensor, the presence of H2O2 was detected in the root hairs by 3,3-diaminobenzidine (DAB) stain 72 h after bacterial inoculation. In-situ pH measurements were also carried out with a gold disc microelectrode modified with a film of iridium oxide and lower pH values were found in A. tequilana leaves treated with bacteria, which may indicate the plant produces acidic substances by biosynthesis of secondary metabolites. This microsensor could be an advantageous tool for further studies on the understanding of the mechanism of H2O2 production during the plant-endophyte interaction. (AU)

FAPESP's process: 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology
Grantee:Paolo Di Mascio
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/22396-6 - Development of a microsensor coupled to scanning electrochemical microscopy to investigate the role of mitochondria on the respiration process
Grantee:Carla Santana Santos
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/02095-4 - Fabrication of nanosensors to be used in scanning electrochemical microscopy and further application in biological systems (single-cell analysis)
Grantee:Alex da Silva Lima
Support type: Scholarships in Brazil - Post-Doctorate