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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MALDI imaging detects endogenous digoxin in glioblastoma cells infected by Zika virusWould it be the oncolytic key?

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Author(s):
Lima, Estela de O. [1] ; Guerreiro, Tatiane M. [1] ; Melo, Carlos Fernando O. R. [1] ; de Oliveira, Diogo N. [1] ; Machado, Daisy [2] ; Lancelloti, Marcelo [2] ; Catharino, Rodrigo R. [1]
Total Authors: 7
Affiliation:
[1] Univ Campinas UNICAMP, Sch Pharmaceut Sci, Innovare Biomarkers Lab, Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Sch Pharmaceut Sci, Biotechnol Lab, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Mass Spectrometry; v. 53, n. 3, p. 257-263, MAR 2018.
Web of Science Citations: 3
Abstract

Recently, microcephaly cases have increased in Americas and have been matter of concern due to Zika virus (ZIKV) recent outbreak. Previous studies have shown that ZIKV-infected progenitor neuronal cells present morphological abnormalities and increased rates of cell death, which may be indicators of microcephaly causes. As recent studies indicate ZIKV' tropism for brain cells, how would a glioblastoma (GBM) lineage behave under ZIKV infection, considering GBM the most common and malignant brain tumor in adults, presenting extreme chemoresistance and high morbidity and mortality rates? The current trend of using genetically engineered oncolytic pathogens as a safe way to eliminate tumors is under development, with trials already in course. Therefore, the present study evaluated the possible oncolytic effects and metabolomic alterations of ZIKV infection at human malignant M059J GBM cells. Microscopic evaluation was performed by using optical microscopy, which showed cytopathic effects induced by ZIKV at GBM cells. For the metabolomics study, both control and infected cell cultures were submitted to matrix laser desorption/ionization mass spectrometry imaging analysis. Mass spectrometry data were submitted to partial least squares discriminant analysis statistical analysis, and distinct biomarkers were elected for each infected groups. This study brings light to unexpectedly induced metabolic changes, as endogenous digoxin as important biomarker for ZIKV-GBM group, associated with cytopathic effects induced by viral infection. These result evidences that genetically engineered ZIKV might be a potential new strategy for neural cancer management through the induction of endogenous digoxin synthesis in GBM cells. (AU)

FAPESP's process: 14/00302-0 - Lipidomics shotgun in giardiasis, meningitis and tuberculosis: the search of diagnostic markers, using revolutionary new techniques of mass spectrometry
Grantee:Cibele Zanardi Esteves
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
Grantee:Aníbal Eugênio Vercesi
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/06809-1 - Determination of chemical markers in various bovine products using mass spectrometry
Grantee:Rodrigo Ramos Catharino
Support type: Regular Research Grants
FAPESP's process: 16/17066-2 - Metabolomics and lipidomics the Zika virus, from mosquitoes to the patient
Grantee:Carlos Fernando Odir Rodrigues Melo
Support type: Scholarships in Brazil - Doctorate