Assessing genetic diversity of Citrus by DArT_seq (TM) genotyping

Author(s)

Sagawa, C. H. D. ^{[1, 2]} ; Cristofani-Yaly, M. ^[2] ; Novelli, V. M. ^[2] ; Bastianel, M. ^[2] ; Machado, M. A. ^[2]

Affiliation

^[1] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 - USA

^[2] Inst Agron, Ctr APTA Citros Sylvio Moreira, Cordeiropolis - Brazil

• Document type: Journal article
• Source: PLANT BIOSYSTEMS; v. 152, n. 4, p. 593-598, 2018.
• Web of Science Citations: 1

Abstract

In citrus despite the diversity among cultivated genotypes related to morphological, physiological, and agronomic traits, low polymorphism is detected at the molecular marker level, and the number of DNA-markers technologies has been insufficient to identify genetic diversity among all citrus varieties. However, DArT\_seq markers can overcome this limitation and identify genetic diversity in several species of citrus. This work developed and applied DArT\_seq platform to study the relationships among species, cultivars, and hybrids of citrus. DArT\_seq yielded a total of 37,260 polymorphic presence/absence markers that generated a dendrogram of similarity among the studied genotypes. The results confirmed the relationships of sweet orange, mandarin, and citron species. DArT\_seq markers showed extensive genetic variation among citrus species and can be applied to Citrus genetic diversity studies. (AU)

• FAPESP's process: 07/08435-5 - Disease resistance in citrus: genetic mapping and functional genomics of new hybrids
• Grantee: Mariângela Cristofani-Yaly
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/18605-0 - Obtaining and evaluation of new varieties of scion and rootstock for citrus
• Grantee: Mariângela Cristofani-Yaly
• Support Opportunities: Research Projects - Thematic Grants