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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storage

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Author(s):
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Hatakeyama, Daniela M. ` ; de Morais-Zani, Karen [1] ; Serino-Silva, Caroline [1] ; Grego, Kathleen F. [1] ; Sant'Anna, Savio S. [1] ; Fernandes, Wilson [1] ; Aniz, Patricia A. E. A. [2] ; Torquato, Ricardo J. S. [3] ; Tanaka, Aparecida S. [3] ; Sanz, Libia [4] ; Calvete, Juan J. [4] ; Tanaka-Azevedo, Anita M. [1]
Total Authors: 12
Affiliation:
[1] Hatakeyama, Daniela M. ', Inst Butantan, Lab Herpetol, Av Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Bacteriol, Av Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Bioquim, Rua 3 Maio 100, BR-04044020 Sao Paulo - Brazil
[4] CSIC, Lab Venom Estruct & Func, Jaime Roig 11, Valencia 46010 - Spain
Total Affiliations: 4
Document type: Journal article
Source: Toxicon; v. 141, p. 34-42, JAN 2018.
Web of Science Citations: 3
Abstract

The number of snakes donated to the Brazilian Instituto Butantan has been decreasing in the past 10 years. This circumstance motivated us to compare the properties of five venom pools of Bothrops jararaca snake stored for up to 54 years. Results showed differences among venom pools regarding enzymatic and other biological activities, such as caseinolytic, phospholipase A(2,) hemorrhagic and coagulant activities, as well as antigenicity. Protein content, reverse-phase chromatographic profile, and immunorecognition by commercial Bothrops antivenom were comparable for all venom pools, although lethality of the most recent preparations was higher. Since the lowest functional activities did not always correspond to older venoms, differences among venom pools used for antivenom production during the period 1963-2008 may correlate with the different proportions of venoms from different localities used in their generation, rather than to long-term storage. We conclude that B. jararaca venoms properly stored for long periods of time retain their structural and pharmacological activities, thus representing useful materials for scientific research and antivenom production. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 14/50510-8 - Comparative study of biomolecules composition of Bothrops jararaca snake venom milked and processed in the herpetology of Butantan Institute between 1963 and 2008
Grantee:Anita Mitico Tanaka-Azevedo
Support type: Regular Research Grants
FAPESP's process: 13/05357-4 - Comparative study of reference Bothrops venom in relation to the venom of Bothrops jararaca born in captivity in herpetology laboratory of the Instituto Butantan
Grantee:Anita Mitico Tanaka-Azevedo
Support type: Regular Research Grants
FAPESP's process: 12/12997-7 - Comparative study of biochemical and biological properties of Bothrops jararaca venoms milked and processed in the Herpetology Laboratory of the Butantan Institute from 1960 to 2008
Grantee:Daniela Miki Hatakeyama
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/10266-5 - Comparative study of composition of biomolecules of Bothrops jararaca venom snake milked and processed in the Herpetology Laboratory of Instituto Butantan between 1963 and 2009
Grantee:Anita Mitico Tanaka-Azevedo
Support type: Regular Research Grants
FAPESP's process: 14/11108-0 - Comparative study of biomolecules composition of Bothrops jararaca and Crotalus durissus terrificus venom born and maintained at the Butantan Institute and newly arrived from nature
Grantee:Karen de Morais Zani
Support type: Regular Research Grants
FAPESP's process: 13/00267-7 - Comparative study of venom composition of Viperidae snakes born and maintained in the Herpetology Laboratory of the Butantan Institute.
Grantee:Karen de Morais Zani
Support type: Scholarships in Brazil - Post-Doctorate