S-nitrosoglutathione-containing chitosan nanoparticles dispersed in Pluronic F-127 hydrogel: Potential uses in topical applications

Author(s)

Pelegrino, Milena T. ^{[1, 2]} ; de Araujo, Daniele R. ^{[1, 2]} ; Seabra, Amedea B. ^{[1, 2]}

Affiliation

^[1] Fed Univ ABC, Ctr Nat & Human Sci, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil

^[2] Fed Univ ABC UFABC, Nanomed Res Unit NANOMED, Santo Andre, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY; v. 43, p. 211-220, FEB 2018.
• Web of Science Citations: 7

Abstract

The aim of this study was to prepare nitric oxide (NO) releasing nanoparticle/hydrogel with potential uses in topical applications. NO is a signaling molecule in skin. It controls the dermal blood flow, skin defense, and tissue repair. There is an interest for designing topical formulations that release NO/NO donors directly at the desired application site. S-nitrosoglutathione (GSNO), a NO donor, was incorporated into chitosan nanoparticles (CS NPs) (hydrodynamic size of 112.2 +/- 2.22 nm). Free GSNO or GSNO-containing CS NPs were incorporated in Pluronic F-127 (PL) hydrogels. The purpose of this study was to combine the advantages of CS NPs (as a drug delivery system with mucoadhesive properties) and a PL hydrogel, which permits a prolonged contact time for the NO donor on the mucosal/skin surfaces. Rheological measurements were performed to determine the sol-gel transition temperature, the rheological parameters, and the viscosity. The polymeric matrices spontaneously released free NO. GSNO diffusion from the polymeric matrices was evaluated using a diffusion system. The results indicate that the incorporation of GSNO-CS NPs into a PL hydrogel may find important dermatological applications due to their mechanical properties and their ability to release therapeutic and sustained amounts of NO at the desired application site. (AU)

• FAPESP's process: 14/13913-7 - Preparation, characterization and biological evaluations of mono and bimetallic nanoparticles systems
• Grantee: Paula Silvia Haddad Ferreira
• Support type: Regular Research Grants
• FAPESP's process: 16/10347-6 - S-nitrosothiol containing nanoparticles: Synthesis, characterization, cytotoxicity and applications
• Grantee: Amedea Barozzi Seabra
• Support type: Regular Research Grants
• FAPESP's process: 15/00393-8 - Nitric oxide releasing polymeric nanoparticles for topical applications
• Grantee: Milena Trevisan Pelegrino
• Support type: Scholarships in Brazil - Master