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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vitro Osteogenesis Stimulation via Nano-Hydroxyapatite/Carbon Nanotube Thin Films on Biomedical Stainless Steel

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Author(s):
Ricci, Ritchelli [3] ; Lobo, Anderson Oliveira [2] ; Marciano, Fernanda Roberta [2]
Total Authors: 3
Affiliation:
[1] Univ Vale Paraiba, Inst Pesquisa Desenvolvimento, Ave Shishima Hifumi 2911, BR-12244000 Sao Paulo - Brazil
[2] Univ Brasil, Inst Ciencia & Tecnol, Rua Carolina da Fonseca 584, BR-08230030 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MATERIALS; v. 11, n. 9 SEP 2018.
Web of Science Citations: 2
Abstract

We evaluated the electrophoretic deposition of nanohydroxyapatite/superhydrop hilic multiwalled carbon nanotube composites (nHAp/MWCNT) onto stainless steel biomedical alloys for applications in bone tissue engineering. First, nHAp/MWCNT composites were dispersed into 0.042 mol.L-1 of Ca(NO3)(2)center dot 4H(2)O + 0.025 mol.L-1 NH4H2PO4 electrolytes (pH = 4.8) at two different concentrations. Next, a voltage of -2 V was applied using 316L stainless steel as a working electrode (0.27 cm(2)), a high-purity platinum coil wire was used as the auxiliary electrode, and an Ag/AgCl (3 M) electrode was used as the reference electrode. The nHAp/MWCNT composites were characterized by transmission electron microscopy. The deposited nHAp and nHAp/MWCNT films were characterized by profilometry, scanning electron microscopy, X-ray diffractometry and Raman spectroscopy. Human osteoblast cells were cultivated with the different materials and in vitro cytotoxicity was evaluated using lactate dehydrogenase (LDH) assay. The osteogenesis process was evaluated by mRNA levels of the three genes that are directly related to bone repair: Alkaline Phosphatase, Osteopontin and Osteocalcin. We showed that rough, crystalline apatite thin films containing phases of nHAp were successfully deposited onto 316L stainless steel alloys. Also, we noticed that nHAp/MWCNT thin films deposited onto 316L stainless steel alloys upregulated the expression of important genes related to bone mineralization and maturation. Our results strongly support the possibility of this new alternative to modify the surface of metallic biomedical alloys to promote bone tissue regeneration. (AU)

FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/12383-5 - In vitro osteogenesis stimulation via nano-hydroxyapatite/carbon nanotube thin films on biomedical stainless steel
Grantee:Fernanda Roberta Marciano
Support Opportunities: Regular Research Grants - Publications - Scientific article
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Research Grants - Young Investigators Grants