| Full text | |
| Author(s): |
Costa, Bruna Z.
[1, 2]
;
Galman, James L.
[1]
;
Slabu, Iustina
[1]
;
France, Scott P.
[1]
;
Marsaioli, Anita J.
[2]
;
Turner, Nicholas J.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs - England
[2] Univ Estadual Campinas, Chem Inst, Rua Monteiro Lobato 277, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | CHEMCATCHEM; v. 10, n. 20, p. 4733-4738, OCT 23 2018. |
| Web of Science Citations: | 6 |
| Abstract | |
A multi-enzymatic cascade process involving transaminases (TAs) and reductive aminases (RedAms) to produce enantiomerically pure 2,5-disubstituted pyrrolidine alkaloids from their respective 1,4-diketones is reported. Several TAs were screened and the best results for diketone monoamination were obtained with an R-selective TA from Mycobacterium chlorophenicum and with an S-selective TA from Bacillus megaterium. Pyrroline reduction was best performed by a reductive aminase from Ajellomyces dermatitidis (AdRedAm). Finally, a biocatalytic one-pot cascade was implemented using the aforementioned enzymes and a variety of 2-methyl-5-alkylpyrrolidines were produced with high (>99%) conversion, diastereomeric and enantiomeric excess values. (AU) | |
| FAPESP's process: | 15/22513-5 - Biocatalytic processes involving transaminases and imine reductases for the synthesis of ant venom alkaloids. |
| Grantee: | Bruna Zucoloto da Costa |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 14/22967-3 - Piperidine and Pyrrolidine Alkaloids Synthesis in Multi-enzymatic systems |
| Grantee: | Bruna Zucoloto da Costa |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock |
| Grantee: | Arlene Gonçalves Corrêa |
| Support Opportunities: | Research Grants - Research Centers in Engineering Program |