Exhaustive acute exercise-induced ER stress is attenuated in IL-6-knockout mice

Author(s):Show less -

Pinto, Ana P. ^[1] ; da Rocha, Alisson L. ^[1] ; Kohama, Eike B. ^[1] ; Gaspar, Rafael C. ^[2] ; Simabuco, Fernando M. ^[2] ; Frantz, Fabiani G. ^[3] ; de Moura, Leandro P. ^[2] ; Pauli, Jose R. ^[2] ; Cintra, Dennys E. ^[2] ; Ropelle, Eduardo R. ^[2] ; de Freitas, Ellen C. ^[4] ; da Silva, Adelino S. R. ^{[4, 1]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of Endocrinology; v. 240, n. 2, p. 181-193, FEB 2019.
• Web of Science Citations: 1

Abstract

The endoplasmic reticulum (ER) stress and inflammation relationship occurs at different levels and is essential for the adequate homeostatic function of cellular systems, becoming harmful when chronically engaged. Intense physical exercise enhances serum levels of interleukin 6 (IL-6). In response to a chronic exhaustive physical exercise protocol, our research group verified an increase of the IL-6 concentration and ER stress proteins in extensor digitorium longus (EDL) and soleus. Based on these results, we hypothesized that IL-6-knockout mice would demonstrate a lower modulation in the ER stress proteins compared to the wild-type mice. To clarify the relationship between exercise-induced IL-6 increased and ER stress, we studied the effects of an acute exhaustive physical exercise protocol on the levels of ER stress proteins in the skeletal muscles of IL-6-knockout (KO) mice. The WT group displayed a higher exhaustion time compared to the IL-6 KO group. After 1 h of the acute exercise protocol, the serum levels of IL-6 and IL-10 were enhanced in the WT group. Independent of the experimental group, the CHOP and cleaved caspase 12/total caspase 12 ratio in EDL as well as ATF6 and CHOP in soleus were sensitive to the acute exercise protocol. Compared to the WT group, the oscillation patterns over time of BiP in EDL and soleus as well as of peIF2-alpha/eIF2-alpha ratio in soleus were attenuated for the IL-6 KO group. In conclusion, IL-6 seems to be related with the ER stress homeostasis, once knockout mice presented attenuation of BiP in EDL and soleus as well as of pEiF2-alpha/EiF2-alpha ratio in soleus after the acute exhaustive physical exercise protocol. (AU)

• FAPESP's process: 17/19869-8 - Molecular mechanisms related to increased hepatic fat content in response to excessive physical exercise
• Grantee: Ana Paula Pinto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/13558-7 - Molecular characterization of S6Ks in obesity and its associated diseases
• Grantee: Fernando Moreira Simabuco
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/12765-2 - Emerging role of rev-erb-alpha in molecular adaptations to different physical exercise models
• Grantee: Alisson Luiz da Rocha
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/09038-1 - EFFECTS OF REGULAR PHYSICAL EXERCISE AND OVERTRAINING ON THE BEHAVIOR OF THE AUTOPHAGIC PATHWAY IN DIFFERENT TISSUES OF MICE.
• Grantee: Adelino Sanchez Ramos da Silva
• Support Opportunities: Regular Research Grants