In vitro SPF and Photostability Assays of Emulsion Containing Nanoparticles with Vegetable Extracts Rich in Flavonoids

Author(s)

Cefali, Leticia Caramori ^{[1, 2]} ; Ataide, Janaina Artem ^{[1, 3]} ; Eberlin, Samara ^[4] ; da Silva Goncalves, Fernanda Cristina ^[4] ; Fernandes, Ana Rita ^[1] ; Marto, Joana ^[5] ; Ribeiro, Helena Margarida ^[5] ; Foglio, Mary Ann ^[6] ; Mazzola, Priscila Gava ^[6] ; Souto, Eliana Barbosa ^{[1, 7]}

Affiliation

^[1] Univ Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, Polo Ciencias Saude, P-3000548 Coimbra - Portugal

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Cidade Univ Zeferino Vaz, Monteiro Lobato St 255, BR-13083862 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Sch Med Sci, R Sergio Buarque de Holanda 250, BR-13083859 Campinas, SP - Brazil

^[4] Kosmosci Grp, Rua Sandoval Meireles 72, BR-13041315 Campinas, SP - Brazil

^[5] UL, Fac Pharm, Res Inst Med iMed ULisboa, P-1649003 Lisbon - Portugal

^[6] Univ Estadual Campinas, UNICAMP, Fac Pharmaceut Sci, Rua Candido Portinari 200, BR-13083871 Campinas, SP - Brazil

^[7] Univ Minho, Ctr Biol Engn, CEB, Campus Gualtar, P-4710057 Braga - Portugal

• Document type: Journal article
• Source: AAPS PHARMSCITECH; v. 20, n. 1 JAN 2019.
• Web of Science Citations: 4

Abstract

The aim of study was to determine the in vitro sun protection factor (SPF) and the photostability profile of a topical formulation composed of nanoparticles loaded with vegetable extracts and to assess its physicochemical properties. Chitosan/tripolyphosphate (TPP) nanoparticles loaded with flavonoids-enriched vegetable extracts (Ginkgo biloba L., Dimorphandra mollis Benth, Ruta graveolens, and Vitis vinifera L.) were produced and characterized for their morphology, mean particle size, zeta potential, and encapsulation efficiency. A final topical formulation was obtained by dispersing chitosan/TPP nanoparticles in an o/w emulsion. Results showed that nanoparticles dispersion exhibited yellowish color, spherical shape, and uniform appearance. Extract-loaded chitosan/TPP nanoparticles showed a mean particle size of 557.11 +/- 3.1nm, polydispersity index of 0.39 +/- 0.27, zeta potential of +11.54 +/- 2.1mV, and encapsulation efficiency of 75.89% of rutin. The recorded texture parameters confirm that the developed formulation is appropriate for skin application. The SPF obtained was 2.3 +/- 0.4, with a critical wavelength of 387.0nm and 0.69 UVA/UVB ratio. The developed formulation exhibited photostability, allowing the release of flavonoids from nanoparticles while retaining rutin into the skin in a higher extension. (AU)

• FAPESP's process: 15/25533-7 - DEVELOPMENT AND EVALUATION OF A FLAVONOID ENRICHED EMULSION WITH SUN PROTECTION FACTOR
• Grantee: Leticia Caramori Cefali
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/14757-7 - Development and evaluation of sun protection factor of emulsion containing extract rich in flavonoids
• Grantee: Leticia Caramori Cefali
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate