Effects of Aerobic Exercise on Molecular Aspects of Asthma: Involvement of SOCS-JAK-STAT

Author(s):Show less -

Almeida-Oliveira, A. R. ^{[1, 2]} ; Aquino, Jr., J. C. J. ^{[1, 2]} ; Abbasi, A. ^[3] ; Santos-Dias, A. ^[2] ; Oliveira, Jr., M. C. ^[2] ; Alberca-Custodio, R. W. ^[2] ; Rigonato-Oliveira, N. C. ^[1] ; Salley-Dias, L. P. ^[4] ; Damaceno-Rodrigues, N. R. ^[5] ; Caldini, E. G. ^[5] ; Arantes-Costa, F. M. ^[6] ; Ligeiro-Oliveira, A. P. ^[1] ; Belvisi, M. G. ^[7] ; Vieira, R. P. ^{[2, 4, 8, 9]}

Affiliation

^[1] Nove de Julho Univ, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Rua Pedro Ernesto 240, BR-12245520 Sao Jose Dos Campos, SP - Brazil

^[3] UCLA Med Ctr, Los Angeles Biomed Res Inst Harbor, Dept Med, Div Resp & Crit Care Physiol & Med, Torrance, CA 90502 - USA

^[4] Univ Brasil, Postgrad Program Bioengn & Biomed Engn, Campus Itaquera, Rua Carolina Fonseca 235, BR-08230030 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Pathol, Lab Cell Biol LIM 59, Ave Doutor Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Sch Med, Dept Clin Med, Lab Expt Therapeut LIM 20, Ave Doutor Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[7] Imperial Coll London, Natl Heart & Lung Inst, Airway Dis, Resp Pharmacol Grp, London - England

^[8] Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Campus Baixada Santista, Av Ana Costa, BR-11060001 Santos, SP - Brazil

^[9] Anhembi Morumbi Univ, Sch Med, Ave Deputado Benedito Matarazzo, 4050 Jardim, BR-12230002 Sao Jose Dos Campos, SP - Brazil

• Document type: Review article
• Source: EXERCISE IMMUNOLOGY REVIEW; v. 25, p. 98-110, 2019.
• Web of Science Citations: 2

Abstract

Background: Aerobic training (AT) decreases airway inflammation in asthma, but the underlying cellular and molecular mechanisms are not completely understood. Thus, this study evaluated the participation of SOCS-JAK-STAT signaling in the effects of AT on airway inflammation, remodeling and hyperresponsiveness in a model of allergic airway inflammation. Methods: C57Bl/6 mice were divided into Control (Co), Exercise (Ex), HDM (HDM), and HDM+ Exercise (HDM+ Ex). Dermatophagoides pteronyssinus (100ug/mouse) were administered oro-tracheally on days 0, 7, 14, 21, 28, 35, 42 and 49. AT was performed in a treadmill during 4 weeks in moderate intensity, from day 24 until day 52. Results: AT inhibited HDM-induced total cells (p<0.001), eosinophils (p<0.01), neutrophils (p<0.01) and lymphocytes (p<0.01) in BAL, and eosinophils (p<0.01), neutrophils (p<0.01) and lymphocytes (p<0.01) in peribronchial space. AT also reduced BAL levels of IL-4 (p<0.001), IL-5 (p<0.001), IL-13 (p<0.001), CXCL1 (p<0.01), IL-17 (p<0.01), IL-23 (p<0.05), IL-33 (p<0.05), while increased IL-10 (p<0.05). Airway collagen fibers (p<0.01), elastic fibers p<0.01) and mucin (p<0.01) were also reduced by AT. AT also inhibited HDM-induced airway hyperresponsiveness (AHR) to methacholine 6,25mg/ml (p<0.01), 12,5mg/mL (p<0.01), 25mg/mL (p<0.01) and 50mg/mL (p<0.01). Mechanistically, AT reduced the expression of STAT6 (p<0.05), STAT3 (p<0.001), STAT5 (p<0.01) and JAK2 (p<0.001), similarly by peribronchial leukocytes and by airway epithelial cells. SOCS1 expression (p<0.001) was upregulated in leukocytes and in epithelial cells, SOCS2 (p<0.01) was upregulated in leukocytes and SOCS3 down-regulated in leukocytes (p<0.05) and in epithelial cells (p<0.001). Conclusions: AT reduces asthma phenotype involving SOCS-JAK-STAT signaling. (AU)

• FAPESP's process: 14/12755-9 - Effects of aerobic physical training in obese asthmatic mice
• Grantee: Jefferson Comin Jonco Aquino Junior
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 12/21519-1 - Is the leukotrienes via contributes to the anti-inflammatory effects of aerobic exercise in asthma?
• Grantee: Ricardo Wesley Alberca Custódio
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 12/16498-5 - Effect of low level laser therapy in experimental models of pulmonary chronic diseases
• Grantee: Ana Paula Ligeiro de Oliveira
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 14/07500-1 - Role of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic exercise in an experimental model of asthma
• Grantee: Ana Roberta Almeida Comin
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support type: Research Grants - Young Investigators Grants