Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection

Author(s)

de Souza, Joao Carlos ^[1] ; da Silva, Jose Luiz ^{[1, 2]} ; Fabrao, Rodrigo Monteiro ^[1] ; Stradiotto, Nelson Ramos ^{[1, 2, 3]} ; Boldrin Zanoni, Maria Valnice ^[1]

Affiliation

^[1] Sao Paulo State Univ Unesp, Inst Chem, Analyt Chem Dept, Natl Inst Alternat Technol Detect Toxicol Assessm, Prof Francisco Degni St 55, BR-14800060 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Bioenergy Res Inst, Prof Francisco Degni St 55, BR-14800060 Araraquara, SP - Brazil

^[3] Fluminense Fed Univ UFF, Sch Ind Engn Met Volta Redonda, Trabalhadores Ave 420, BR-27255125 Volta Redonda, RJ - Brazil

• Document type: Journal article
• Source: Microchemical Journal; v. 147, p. 972-978, JUN 2019.
• Web of Science Citations: 1

Abstract

The present work describes an easy, rapid and sensitive alternative methodology for simultaneous detection of sugars (glucose, fructose and arabinose), organic acids (gluconic, glucuronic and glucaric acids) and sugar alcohol (arabitol) in wine samples. The method is based on high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) at gold electrode under detection potential of 0.26 V vs Pd/PdO. The optimized chromatographic anion-exchange separation indicates good separation and detection using a binary gradient with 0.28 mol L-1 CH3COONa containing 0.10 mol L-1 NaOH and ultrapure water as mobile phase with a flow rate at 1.0 mL min(-1). Analytical curves presented linear relationship from 1.0 x 10(-7) and 1.0 x 10(-3) mol L-1 with detection limits (S/N = 3) from 3.0 x 10(-8) (glucose) to 9.0 x 10(-7) mol L-1 (gluconic acid). The present method was successfully applied in the determination of sugars, organic acids and sugar alcohols in wine, without time consuming pre-treatment of the sample, no need for organic solvent, fast relative execution time, satisfactory separation, high sensitivity and accuracy. (AU)

• FAPESP's process: 14/03679-7 - Degradation of hair dyes and salon wastewater by photoelectrocatalysis with modified TiO2 nanotube electrodes and combination with ozonation
• Grantee: Guilherme Garcia Bessegato
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/12998-1 - Chromatographic and electroanalytical methods applied in determination of p-toluenediamine and p-aminophenol in oxidative medium used in permanent hair dye.
• Grantee: João Carlos de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate