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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Purification and Physicochemical Characterization of a Novel Thermostable Xylanase Secreted by the Fungus Myceliophthora heterothallica F.2.1.4

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Author(s):
de Oliveira Simoes, Lorena Caixeta [1] ; da Silva, Ronivaldo Rodrigues [1] ; de Oliveira Nascimento, Carlos Eduardo [1] ; Boscolo, Mauricio [1] ; Gomes, Eleni [1] ; da Silva, Roberto [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ Unesp, Inst Biosci Human & Exact Sci, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Applied Biochemistry and Biotechnology; v. 188, n. 4, p. 991-1008, AUG 2019.
Web of Science Citations: 3
Abstract

Xylanases are enzymes that act in the depolymerization of xylan and that can be used in the food industry, the paper industry, and for bioenergy, among other uses. In this context, particular emphasis is devoted to xylooligosaccharides (XOS) that act as prebiotics, which, under the action of probiotic microorganisms, are capable of positively modifying the intestinal microbiota. In this sense, searching for microbial xylanases stands out as a sustainable strategy for the production of prebiotics. To date, there have been no reports in the literature regarding the purification of native xylanase from Myceliophthora heterothallica F.2.1.4. In this study, a xylanase from this fungus was purified and characterized. The xylanase, with 27kDa, showed maximum activity at pH4.5 and 65-70 degrees C. It maintained more than 80% of its residual activity when exposed to (i) temperatures between 30 and 60 degrees C for 1h and (ii) pH5-10 for 24h at 4 and 25 degrees C. These high tolerances to different pH and different temperatures are important properties that add value to this enzyme. The hydrolysates of this enzyme on beechwood xylan, analyzed by HPAE-PAD, were mostly xylobiose (X2) and xylotriose (X3). Hydrolysates were also quantified, being retrieved from 234.2mg xylooligosaccharides/g of hydrolyzed xylan for 12h. According to the products obtained from the xylan hydrolysis and its tolerance properties of the enzyme, it has demonstrated potential for application production of xylooligosaccharides for use as prebiotics. (AU)

FAPESP's process: 17/16482-5 - Purification and immobilization of beta-glucosidase from Thermoascus aurantiacus using supermacroporous cryogels and application in bioprocesses
Grantee:Roberto da Silva
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants