Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination

Author(s):Show less -

de Lima, Diogenes S. ^[1] ; Cardozo, Lucas E. ^[1] ; Maracaja-Coutinho, Vinicius ^[2] ; Suhrbier, Andreas ^[3] ; Mane, Karim ^[4] ; Jeffries, David ^[4] ; Silveira, Eduardo L. V. ^[1] ; Amaral, Paulo P. ^[5] ; Rappuoli, Rino ^{[6, 7]} ; de Silva, Thushan I. ^{[4, 8, 9]} ; Nakaya, Helder I. ^{[1, 10]}

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo - Brazil

^[2] Univ Chile, Fac Ciencias Quim & Farmaceut, Adv Ctr Chron Dis ACCDiS, Santiago 8380492 - Chile

^[3] QIMR Berghofer Med Res Inst, Inflammat Biol Lab, Brisbane, Qld 4029 - Australia

^[4] Gambia LSHTM, Vaccines & Immun Theme Med Res Council Unit, Banjul - Gambia

^[5] Univ Cambridge, Gurdon Inst, Cambridge CB2 1QN - England

^[6] GlaxoSmithKline, I-53100 Siena - Italy

^[7] Imperial Coll London, Dept Infect Dis, London W12 ONN - England

^[8] Imperial Coll London, Dept Med, Ctr Int Child Hlth, Paediat Sect, London W2 1PG - England

^[9] Univ Sheffield, Dept Infect Immun & Cardiovasc Dis, Sheffield S10 2RX, S Yorkshire - England

^[10] Univ Sao Paulo, Sci Platform Pasteur, BR-05508210 Sao Paulo - Brazil

• Document type: Journal article
• Source: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 116, n. 34, p. 17121-17126, AUG 20 2019.
• Web of Science Citations: 1

Abstract

Understanding the mechanisms of vaccine-elicited protection contributes to the development of new vaccines. The emerging field of systems vaccinology provides detailed information on host responses to vaccination and has been successfully applied to study the molecular mechanisms of several vaccines. Long noncoding RNAs (lncRNAs) are crucially involved in multiple biological processes, but their role in vaccine-induced immunity has not been explored. We performed an analysis of over 2,000 blood transcriptome samples from 17 vaccine cohorts to identify lncRNAs potentially involved with antibody responses to influenza and yellow fever vaccines. We have created an online database where all results from this analysis can be accessed easily. We found that lncRNAs participate in distinct immunological pathways related to vaccine-elicited responses. Among them, we showed that the expression of lncRNA FAM30A was high in B cells and correlates with the expression of immunoglobulin genes located in its genomic vicinity. We also identified altered expression of these lncRNAs in RNA-sequencing (RNA-seq) data from a cohort of children following immunization with intranasal live attenuated influenza vaccine, suggesting a common role across several diverse vaccines. Taken together, these findings provide evidence that lncRNAs have a significant impact on immune responses induced by vaccination. (AU)

• FAPESP's process: 12/19278-6 - Systems biology of long non-coding RNAs
• Grantee: Helder Takashi Imoto Nakaya
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/50137-3 - Long noncoding RNA interplay with the host microbiome may determine mucosal influenza vaccine immunogenicity
• Grantee: Helder Takashi Imoto Nakaya
• Support Opportunities: Regular Research Grants